Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 West Youyi Road, 710072 Xi'an, China; Shaanxi Key Laboratory of Integrated Traditional and Western Medicine for Prevention and Treatment of Cardiovascular Diseases, Institute of Integrative Medicine, Shaanxi University of Chinese Medicine, Shiji Ave., 712046, Xi'an-Xianyang New Ecomic Zone, China.
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 West Youyi Road, 710072 Xi'an, China.
Int J Biol Macromol. 2021 Dec 15;193(Pt B):2021-2028. doi: 10.1016/j.ijbiomac.2021.11.032. Epub 2021 Nov 10.
Mg alloys are increasingly being investigated as a versatile and economical alternative for developing bone repair implants because of their high mechanical strength, wide availability, adjustable structure and properties. In this study, magnesium alloy WE43 is coated on both sides with gelatin nanosphere/chitosan (GNs/CTS), a coating enhanced by incorporating simvastatin (SIM). SIM-loaded GNs/CTS coated magnesium alloy can promote the osteogenic differentiation of bone mesenchymal stem cells (BMSCs). BMSCs and human umbilical vein endothelial cells (HUVECs) are co-cultured through transwell systems. The release of SIM from the coating is found to increase the secretion of chemokine and angiogenic factors from BMSCs, which promote the migration and tube formation of HUVECs, respectively. Bone morphogenetic protein secreted by HUVECs is seen to increase by the release of SIM from the coating, promoting the osteogenic differentiation of BMSCs. The secretion of chemokines from HUVECs promote the migration of BMSCs. The coated magnesium alloy substrate loaded with SIM is found to regulate the osteogenic differentiation of BMSCs. The study of the paracrine interaction between BMSCs and HUVECs proves that the applied coating promotes both osteogenic differentiation and vascularization, thus demonstrating a new approach for the design of bone repair materials based on magnesium alloys.
镁合金由于其高强度、广泛的可用性、可调节的结构和性能，正作为一种通用且经济的替代方案，越来越多地被用于开发骨修复植入物。在这项研究中，镁合金 WE43 的两面都涂有明胶纳米球/壳聚糖（GNs/CTS）涂层，通过掺入辛伐他汀（SIM）对其进行了增强。载 SIM 的 GNs/CTS 涂层镁合金可以促进骨髓间充质干细胞（BMSCs）的成骨分化。通过 Transwell 系统共培养 BMSCs 和人脐静脉内皮细胞（HUVECs）。研究发现，涂层中 SIM 的释放会增加 BMSCs 分泌趋化因子和血管生成因子，分别促进 HUVECs 的迁移和管形成。HUVECs 分泌的骨形态发生蛋白通过涂层中 SIM 的释放而增加，从而促进 BMSCs 的成骨分化。HUVECs 分泌的趋化因子促进 BMSCs 的迁移。载 SIM 的涂层镁合金基底被发现可调节 BMSCs 的成骨分化。对 BMSCs 和 HUVECs 之间旁分泌相互作用的研究证明，所应用的涂层促进了成骨分化和血管生成，从而为基于镁合金的骨修复材料的设计提供了一种新方法。
