Mazzotta Marta, Salaris Silvano, Pascale Maria Rosaria, Girolamini Luna, Cristino Sandra
Department of Biological, Geological, and Environmental Sciences, University of Bologna, via San Giacomo 12, 40126 Bologna, Italy.
Pathogens. 2021 May 3;10(5):552. doi: 10.3390/pathogens10050552.
species distribution in the Emilia-Romagna region, involving hospital (H) and community (C) environments, was conducted. culture, agglutination test, and -gene sequencing were applied on 240 isolates. The analysis showed a higher prevalence of non- (n-) species (84.1%) compared with () (15.9%), with a higher frequency of n- with respect to species in both environments (77.6% and 96.4%, in H and C, respectively). The Shannon index showed a significant difference in distribution ( = 0.00017), with a significant abundance of in the H compared with C environment ( = 0.00028). The continuous disinfection treatment in H could contribute to adaptive survival of the species. Phylogenetic analysis revealed a conservative clade distribution between H and C: clade with three subclades in C and the clade with five subclades in H and two in C, respectively. Our findings suggest the importance of surveillance both in H and C, with a focus on n- species less connected to human disease. The prevalence and diversity found here indicate that geographical and temporal isolate evolution should be considered during surveillance, particularly in the light of global warming and changes in population risk factors.
对艾米利亚 - 罗马涅地区的物种分布进行了研究，涉及医院（H）和社区（C）环境。对240株分离株进行了培养、凝集试验和基因测序。分析表明，非（n -）物种的患病率（84.1%）高于物种（15.9%），在两种环境中n -物种的频率均高于物种（在医院环境中为77.6%，在社区环境中为96.4%）。香农指数显示物种分布存在显著差异（P = 0.00017），与社区环境相比，医院环境中物种的丰度显著更高（P = 0.00028）。医院中的持续消毒处理可能有助于物种的适应性生存。系统发育分析揭示了医院和社区之间保守的进化枝分布：社区中有一个进化枝和三个亚进化枝，医院中有一个进化枝和五个亚进化枝，社区中有两个亚进化枝。我们的研究结果表明，在医院和社区中进行监测都很重要，重点是与人类疾病关联较少的n -物种。这里发现的物种患病率和多样性表明，在监测过程中应考虑地理和时间上分离株的进化，特别是鉴于全球变暖和人群风险因素的变化。
