Characterization of a Novel Species of Isolated from a Healthcare Facility: sp. nov.

Two -like isolates, 8cVS16 and 9fVS26, were isolated from a water distribution system (WDS) in a healthcare facility. Cells were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, motile, and exhibited a blue-white fluorescence under Wood's lamp at 365 nm. The strains grew in a range of 32-37 °C on BCYE with (C+), GVPC, and MWY agar medium, with a positive reaction for oxidase, catalase, and gelatinase. The dominant fatty acids were summed features 3 (Cω7c/Cω6c) (27.7%), C iso (17.5%), and C (16.3%), and Q13 as the major ubiquinone. The and gene sequences showed a similarity of 96.7% and 92.4%, with (ATCC 35292). The whole genomes sequencing (WGS) performed displayed a GC content of 38.21 mol% for both. The digital DNA-DNA hybridization (dDDH) analysis demonstrated the separation of the two strains from the phylogenetically most related ATCC 35292), with ≤43% DNA-DNA relatedness. The Average Nucleotide Identity (ANI) between the two strains and (ATCC 35292) was 90.74%, confirming that the two isolates represent a novel species of the genus . The name proposed for this species is sp. nov., with 8cVS16 (=DSM 114356 = CCUG 76627) as the type strain.