Shaqour Bahaa, Aizawa Juliana, Guarch-Pérez Clara, Górecka Żaneta, Christophersen Lars, Martinet Wim, Choińska Emilia, Riool Martijn, Verleije Bart, Beyers Koen, Moser Claus, Święszkowski Wojciech, Zaat Sebastian A J, Cos Paul
Laboratory for Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1 S.7, 2610 Wilrijk, Belgium.
Mechanical and Mechatronics Engineering Department, Faculty of Engineering & Information Technology, An-Najah National University, Nablus P.O. Box 7, Palestine.
Pharmaceutics. 2021 May 21;13(6):772. doi: 10.3390/pharmaceutics13060772.
Additive manufacturing is widely used to produce highly complex structures. Moreover, this technology has proven its superiority in producing tools which can be used in different applications. We designed and produced an extrusion nozzle that allowed us to hot melt extrude drug-loaded tubes. The tubes were an essential part of a new mouse ventilator-associated pneumonia (VAP) model. Ciprofloxacin (CPX) was selected for its expected activity against the pathogen and ease of incorporation into thermoplastic polyurethane (TPU). TPU was selected as the carrier polymer for its biocompatibility and use in a variety of medical devices such as tubing and catheters. The effect of loading CPX within the TPU polymeric matrix and the physicochemical properties of the produced tubes were investigated. CPX showed good thermal stability and in vitro activity in preventing biofilm formation after loading within the tube's polymeric matrix. Moreover, the produced tubes showed anti-infective efficacy in vivo. The produced tubes, which were extruded via our novel nozzle, were vital for the validation of our mouse VAP model. This model can be adopted to investigate other antibacterial and antibiofilm compounds incorporated in polymeric tubes using hot melt extrusion.
增材制造被广泛用于生产高度复杂的结构。此外，这项技术在生产可用于不同应用的工具方面已证明了其优越性。我们设计并生产了一种挤出喷嘴，使我们能够热熔挤出载药管。这些管子是一种新型小鼠呼吸机相关性肺炎（VAP）模型的重要组成部分。选择环丙沙星（CPX）是因为其对病原体具有预期的活性且易于掺入热塑性聚氨酯（TPU）中。选择TPU作为载体聚合物是因为其具有生物相容性且可用于各种医疗设备，如管材和导管。研究了在TPU聚合物基质中负载CPX的效果以及所生产管子的物理化学性质。CPX在负载于管子的聚合物基质后表现出良好的热稳定性和体外预防生物膜形成的活性。此外，所生产的管子在体内显示出抗感染功效。通过我们的新型喷嘴挤出的管子对于我们小鼠VAP模型的验证至关重要。该模型可用于研究使用热熔挤出法掺入聚合物管中的其他抗菌和抗生物膜化合物。
