Soman Soja Saghar, Govindraj Mano, Al Hashimi Noura, Zhou Jiarui, Vijayavenkataraman Sanjairaj
The Vijay Lab, Division of Engineering, New York University Abu Dhabi, Abu Dhabi, UAE.
Department of Mechanical and Aerospace Engineering, Tandon School of Engineering, New York University, Brooklyn, USA.
Int J Bioprint. 2022 Aug 23;8(4):604. doi: 10.18063/ijb.v8i4.604. eCollection 2022.
Bioprinting of nervous tissue is a major challenge in the bioprinting field due to its soft consistency and complex architecture. The first step in efficient neural bioprinting is the design and optimization of printable bioinks which favor the growth and differentiation of neural tissues by providing the mechanophysiological properties of the native tissue microenvironment. However, till date, limited studies have been conducted to make tissue specific bioinks. Here, we report a novel bioink formulation specifically designed for bioprinting and differentiation of neural stem cells (NSCs) to peripheral neurons, using a marine tunicate-derived hydrogel and Matrigel. The formulation resulted in seamless bioprinting of NSCs with minimal processing time from bioink preparation to culture. The tissues exhibited excellent post-printing viability and cell proliferation along with a precise peripheral nerve morphology on differentiation. The cultured tissues showed significant cell recovery after subjecting to a freeze-thaw cycle of -80 to 37°C, indicating the suitability of the method for developing tissues compatible for long-term storage and transportation for clinical use. The study provides a robust method to use a sustainable bioink for three-dimensional bioprinting of neural tissues for translational medicine applications.
由于神经组织质地柔软且结构复杂，神经组织的生物打印是生物打印领域的一项重大挑战。高效神经生物打印的第一步是设计和优化可打印生物墨水，通过提供天然组织微环境的机械生理特性来促进神经组织的生长和分化。然而，迄今为止，针对制备组织特异性生物墨水的研究还很有限。在此，我们报告了一种新型生物墨水配方，该配方专门设计用于将神经干细胞（NSCs）生物打印并分化为外周神经元，使用的是源自海洋被囊动物的水凝胶和基质胶。该配方实现了神经干细胞的无缝生物打印，从生物墨水制备到培养的处理时间最短。这些组织在分化后表现出优异的打印后活力和细胞增殖能力，以及精确的外周神经形态。在经历 -80 至 37°C 的冻融循环后，培养的组织显示出显著的细胞恢复，这表明该方法适用于开发适合临床长期储存和运输的组织。该研究提供了一种强大的方法，可使用可持续生物墨水进行神经组织的三维生物打印，用于转化医学应用。
