Apffel Alex, Zhao Limian, Sartain Mark J
Agilent Technologies, Santa Clara, CA 95051, USA.
Agilent Technologies, Wilmington, DE 19808, USA.
Metabolites. 2021 May 4;11(5):294. doi: 10.3390/metabo11050294.
Lipidomic approaches are widely used to investigate the relationship between lipids, human health, and disease. Conventional sample preparation techniques for the extraction of lipids from biological matrices like human plasma are based on liquid-liquid extraction (LLE). However, these methods are labor-intensive, time-consuming, and can show poor reproducibility and selectivity on lipid extraction. A novel, solid-phase extraction (SPE) approach was demonstrated to extract lipids from human plasma using a lipid extraction SPE in both cartridge and 96-well-plate formats, followed by analysis using a combination of targeted and untargeted liquid chromatography/mass spectrometry. The Lipid Extraction SPE method was compared to traditional LLE methods for lipid class recovery, lipidome coverage, and reproducibility. The novel SPE method used a simplified protocol with significant time and labor savings and provided equivalent or better qualitative and quantitative results than traditional LLE methods with respect to several critical performance metrics; recovery, reproducibility, and lipidome coverage.
脂质组学方法被广泛用于研究脂质、人类健康和疾病之间的关系。从人血浆等生物基质中提取脂质的传统样品制备技术基于液-液萃取（LLE）。然而，这些方法劳动强度大、耗时，并且在脂质提取方面可能表现出较差的重现性和选择性。一种新型的固相萃取（SPE）方法被证明可使用脂质提取SPE从人血浆中提取脂质，该方法有柱式和96孔板两种形式，随后使用靶向和非靶向液相色谱/质谱联用进行分析。将脂质提取SPE方法与传统LLE方法在脂质类别回收率、脂质组覆盖范围和重现性方面进行了比较。这种新型SPE方法采用了简化方案，显著节省了时间和人力，并且在几个关键性能指标（回收率、重现性和脂质组覆盖范围）方面提供了与传统LLE方法相当或更好的定性和定量结果。
