Artemis One Health Research Foundation, Delft, the Netherlands.
HKU-Pasteur Research Pole, School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
Biochimie. 2020 Dec;179:229-236. doi: 10.1016/j.biochi.2020.10.010. Epub 2020 Oct 25.
The ongoing pandemic of COVID-19 (Coronavirus Disease-2019), a respiratory disease caused by the novel coronavirus strain, SARS-CoV-2, has affected more than 42 million people already, with more than one million deaths worldwide (as of October 25, 2020). We are in urgent need of therapeutic interventions that target the host-virus interface, which requires a molecular understanding of the SARS-CoV-2 life-cycle. Like other positive-sense RNA viruses, coronaviruses remodel intracellular membranes to form specialized viral replication compartments, including double-membrane vesicles (DMVs), where viral RNA genome replication takes place. Here we review the current knowledge of the structure, lipid composition, function, and biogenesis of coronavirus-induced DMVs, highlighting the druggable viral and cellular factors that are involved in the formation and function of DMVs.
持续的 COVID-19（新型冠状病毒肺炎）大流行是由新型冠状病毒株 SARS-CoV-2 引起的呼吸道疾病，已经影响了超过 4200 万人，全球有超过 100 万人死亡（截至 2020 年 10 月 25 日）。我们迫切需要针对宿主-病毒界面的治疗干预措施，这需要对 SARS-CoV-2 生命周期有分子水平的理解。像其他正链 RNA 病毒一样，冠状病毒重塑细胞内膜以形成专门的病毒复制隔室，包括双膜囊泡（DMVs），病毒 RNA 基因组复制在此发生。本文综述了冠状病毒诱导的 DMVs 的结构、脂质组成、功能和生物发生的最新知识，重点介绍了参与 DMVs 形成和功能的可成药的病毒和细胞因子。
