Cyclotron and Radiochemistry Program, Radiology Department, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Bone-Muscle Research Center, College of Nursing and Health Innovation, The University of Texas at Arlington, Arlington, TX, USA.
Methods Mol Biol. 2024;2816:151-159. doi: 10.1007/978-1-0716-3902-3_15.
Developing robust analytical techniques is a vital phase to facilitate understanding the roles and impacts of various omic profilings in cellular functions. The comprehensive analysis of various biological molecules within a biological system requires a precise sample preparation technique. Solid-Phase Extraction (SPE) has proven to be an indispensable method in lipidomic analysis, providing an uncomplicated and user-friendly technique for extraction and purification of lipid components from complex biological matrices. Of all the factors influencing the reliability and success of SPE, column or adsorbent materials, flow rate, and storage conditions are paramount in terms of their significance. In this chapter, we will discuss in detail the SPE steps for lipidomic analysis in biofluid samples (serum and plasma) and muscle tissues.
开发强大的分析技术是促进理解各种组学在细胞功能中的作用和影响的重要阶段。在生物系统中对各种生物分子进行综合分析需要精确的样品制备技术。固相萃取 (SPE) 已被证明是脂质组学分析中不可或缺的方法，它为从复杂的生物基质中提取和纯化脂质成分提供了一种简单易用的技术。在影响 SPE 可靠性和成功的所有因素中，柱或吸附剂材料、流速和储存条件在重要性方面至关重要。在本章中，我们将详细讨论生物流体样品（血清和血浆）和肌肉组织中脂质组学分析的 SPE 步骤。
