Department of Microbiology, Faculty of Agriculture, University of Zagreb, 10000 Zagreb, Croatia.
Department of Fisheries, Apiculture, Wildlife Management and Special Zoology, Faculty of Agriculture, University of Zagreb, 10000 Zagreb, Croatia.
Molecules. 2021 May 20;26(10):3049. doi: 10.3390/molecules26103049.
The incidence of antibiotic resistance in pathogenic bacteria has become an alarming clinical and social problem. Therefore, the demand for alternative antimicrobial compounds has increased. In this study, a chemical profile of honey bee ( L.) venom (HBV) has been determined by HPLC and FTIR-ATR spectroscopy, and tested for antibacterial activity, as well as efficiency with regard to conventional antibiotics. The investigated HBV was of high quality with melittin and total protein contents of 70.10 ± 7.01%, and 84.44 ± 3.12 g/100 g, respectively. The purity of HBV was confirmed by FTIR-ATR spectral profiling, which revealed a unique pattern of absorption bands that are characteristic of its major fractions. In addition, HBV showed a broad spectrum of activity against all three tested biomasses of potentially pathogenic Gram-positive and Gram-negative bacteria with MIC values ranging between 12.5 and 200 µg/mL, and MBC between 12.5 and 400 µg/mL. When compared to conventional antibiotics, HBV (400 µg) showed up to 27.8% efficiency of tetracycline (30 µg), 52.2% erythromycin (15 µg), 21.2% ciprofloxacin (5 µg), and 34.6% of ampicillin-sulbactam (20 µg). The overall results demonstrate the therapeutic potential of the analyzed HBV.
蜂毒（HBV）的化学特征通过 HPLC 和 FTIR-ATR 光谱法进行了确定，并对其抗菌活性以及与常规抗生素的效率进行了测试。研究发现，HBV 质量很高，其蜂毒素和总蛋白含量分别为 70.10±7.01%和 84.44±3.12g/100g。FTIR-ATR 光谱特征分析证实了 HBV 的纯度，其独特的吸收带模式是其主要成分的特征。此外，HBV 对所有三种测试的潜在致病性革兰氏阳性和革兰氏阴性细菌生物量均表现出广谱活性，其 MIC 值在 12.5 至 200μg/mL 之间，MBC 值在 12.5 至 400μg/mL 之间。与常规抗生素相比，HBV（400μg）对四环素（30μg）的效率高达 27.8%，对红霉素（15μg）的效率为 52.2%，对环丙沙星（5μg）的效率为 21.2%，对氨苄西林-舒巴坦（20μg）的效率为 34.6%。总体结果表明，分析的 HBV 具有治疗潜力。
