Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 13, 10-719 Olsztyn, Poland.
Department of Parasitology and Invasive Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego St. 13, 10-719 Olsztyn, Poland.
Molecules. 2021 May 8;26(9):2786. doi: 10.3390/molecules26092786.
The effect of effective microorganisms (EM) on internal organ morphology, intestinal morphometry, and serum biochemical activity in Japanese quails under challenge was determined. After 30 days of EM addition, one group of quails was orally inoculated with . The second group did not receive EM and was inoculated with . In the gut, EM supplementation reduced the number of lesions, enhanced gut health, and protected the mucosa from pathogenic bacteria. EM showed an anti-inflammatory effect and fewer necrotic lesions in villi. In the internal organs, EM showed a protective effect against a typical lesion of infection. Necrosis and degeneration of the hepatocytes, necrosis of bile ducts, and bile duct proliferation were more severe in the infected group without EM. Morphometric evaluation showed significantly higher villi in the jejunum after EM addition. A greater crypt depth was observed in the group. Biochemical analysis of the blood indicated lower cholesterol on the 12th day of the experiment and between-group differences in total protein, lactate dehydrogenase (LDH), and albumin levels in the EM group. Further studies are needed to improve EM activity against pathologic bacteria as a potential alternative to antibiotics and to develop future natural production systems.
本研究旨在探讨有效微生物（Effective microorganisms，EM）对感染鸡体内器官形态、肠道形态计量学和血清生化活性的影响。在 EM 添加 30 天后，一组鹌鹑经口接种 ，另一组未添加 EM 并接种 。在肠道中，EM 补充剂减少了病变数量，增强了肠道健康，并保护了黏膜免受病原菌的侵害。EM 表现出抗炎作用，绒毛中的坏死病变较少。在内部器官中，EM 对 的典型病变表现出保护作用。未添加 EM 的感染组中，肝细胞坏死和变性、胆管坏死和胆管增生更为严重。形态计量学评估显示，添加 EM 后空肠的绒毛高度显著增加。EM 组的隐窝深度更大。血液生化分析表明，实验第 12 天的胆固醇水平较低，EM 组的总蛋白、乳酸脱氢酶（lactate dehydrogenase，LDH）和白蛋白水平存在组间差异。需要进一步研究以提高 EM 对病原菌的活性，作为抗生素的替代物，并开发未来的天然生产系统。
