Gkikas Fedon-Ioannis, Tako Alexandros, Gkizi Danai, Lagogianni Christina, Markakis Emmanouil A, Tjamos Sotirios E
Laboratory of Plant Pathology, Agricultural University of Athens, 75 Iera Odos str., 11855 Athens, Greece.
Laboratory of Mycology, Department of Viticulture, Vegetable Crops, Floriculture and Plant Protection, Hellenic Agricultural Organization Demeter, Mesa Katsampas, 71003 Iraklio Crete, Greece.
Plants (Basel). 2021 Jan 22;10(2):207. doi: 10.3390/plants10020207.
In the last two decades grapevine trunk diseases (GTDs) have emerged as the most significant threat for grapevine sustainability worldwide. The tracheomycotic fungus (Pch) is the predominant GTD-associated species and cannot be controlled with available chemicals. In the present study, we evaluated the effectiveness of two microbial strains ( K165 and F2) against Pch in grapevine. In vitro bioassays, performed in a growth culture medium simulating the xylem environment, indicated that F2 decreased Pch growth and sporulation, whereas K165 did not have any effect on Pch growth. experiments revealed that root-drench and stem-puncture application of K165 and F2 reduced the endophytic relative DNA amount of Pch by 90% and 82%, respectively, compared to controls. However, wood discoloration, the typical symptom of Pch infection, was not reduced in the F2 treated grapevines. Nevertheless, the F2 treated grapevines harbored higher lignin levels compared to mocks, as it was also done by K165. Therefore, F2 and K165 have the potential to be used as biocontrol agents against Pch in grapevines.
在过去二十年中，葡萄树干病害（GTDs）已成为全球葡萄可持续发展面临的最重大威胁。气管真菌（Pch）是与GTD相关的主要菌种，现有化学药剂无法对其进行防治。在本研究中，我们评估了两种微生物菌株（K165和F2）对葡萄中Pch的防治效果。在模拟木质部环境的生长培养基中进行的体外生物测定表明，F2可抑制Pch的生长和孢子形成，而K165对Pch的生长没有任何影响。实验表明，与对照相比，对K165和F2进行根灌和茎穿刺处理后，Pch的内生相对DNA量分别减少了90%和82%。然而，在经F2处理的葡萄藤中，并未减轻Pch感染的典型症状——木材变色。尽管如此，与 mock处理相比，经F2处理的葡萄藤中木质素水平更高，K165处理的情况也是如此。因此，F2和K165有潜力用作葡萄中Pch的生物防治剂。
