de la Huerta-Bengoechea Paula, Gil-Serna Jéssica, Melguizo Clara, Ramos Antonio J, Prim Montserrat, Vázquez Covadonga, Patiño Belén
Department of Genetics, Physiology and Microbiology, Faculty of Biology, University Complutense of Madrid, Jose Antonio Novais 12, 28040 Madrid, Spain.
Agrotecnio-Cerca Center, Food Technology Department, University of Lleida, Rovira Roure 191, 25198 Lleida, Spain.
J Fungi (Basel). 2022 Oct 27;8(11):1136. doi: 10.3390/jof8111136.
The presence of mycotoxin-producing species in vineyards is a problem for food safety and the economy. In addition, rising temperatures due to climate change are modifying microbial communities, causing the replacement of some fungal species and the rise of mycotoxins such as aflatoxins. The use of microorganisms as biological control agents (BCAs) is one of the most promising strategies to prevent fungal growth and toxin production. In this study, 513 microorganisms were isolated from organic vineyard soils in different regions of Spain. The 480 bacteria and 33 yeasts isolated were sequentially screened to select those with the most suitable characteristics to be used as BCAs. After identifying 16 isolates meeting all requirements, six bacterial isolates were selected to test their potential to control three relevant toxigenic grape fungi in vitro: , and . Isolates of sp., sp. and showed an excellent ability to reduce the growth and mycotoxin concentration of the above-mentioned fungi and represent potential candidates for further study regarding their possible industrial application as a BCA.
葡萄园中产生霉菌毒素的物种的存在对食品安全和经济来说是个问题。此外，气候变化导致的气温上升正在改变微生物群落，致使一些真菌物种被取代，黄曲霉毒素等霉菌毒素增加。使用微生物作为生物防治剂（BCA）是防止真菌生长和毒素产生的最有前景的策略之一。在本研究中，从西班牙不同地区的有机葡萄园土壤中分离出513种微生物。对分离出的480株细菌和33株酵母菌进行了顺序筛选，以选择具有最适合特性的用作生物防治剂的菌株。在鉴定出16株符合所有要求的分离株后，选择了6株细菌分离株在体外测试它们控制三种相关产毒葡萄真菌的潜力： 、 和 。 属、 属和 的分离株显示出优异的降低上述真菌生长和霉菌毒素浓度的能力，并且是关于其作为生物防治剂可能的工业应用进行进一步研究的潜在候选菌株。
