Aliyu Habibu, Kastner Ronnie, Maayer Pieter de, Neumann Anke
Section II: Technical Biology, Institute of Process Engineering in Life Science, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany.
School of Molecular & Cell Biology, Faculty of Science, University of the Witwatersrand, Johannesburg 2000, South Africa.
Microorganisms. 2021 May 19;9(5):1090. doi: 10.3390/microorganisms9051090.
is known to catalyse the biological water gas shift (WGS) reaction, a pathway that serves as a source of alternative energy and carbon to a wide variety of bacteria. Despite increasing interest in this bacterium due to its ability to produce biological hydrogen through carbon monoxide (CO) oxidation, there are no data on the effect of toxic CO gas on its physiology. Due to its general requirement of O, the organism is often grown aerobically to generate biomass. Here, we show that carbon monoxide (CO) induces metabolic changes linked to distortion of redox balance, evidenced by increased accumulation of organic acids such as acetate and lactate. This suggests that survives by expressing several alternative pathways, including conversion of pyruvate to lactate, which balances reducing equivalents (oxidation of NADH to NAD), and acetyl-CoA to acetate, which directly generates energy, while CO is binding terminal oxidases. The data also revealed clearly that gained energy and grew during the WGS reaction. Combined, the data provide critical information essential for further development of the biotechnological potential of .
已知其能催化生物水煤气变换（WGS）反应，这是一条为多种细菌提供替代能源和碳源的途径。尽管由于其通过一氧化碳（CO）氧化产生生物氢的能力，人们对这种细菌的兴趣与日俱增，但关于有毒CO气体对其生理影响的数据却尚无报道。由于其对氧气的普遍需求，该生物体通常在有氧条件下培养以产生生物质。在此，我们表明一氧化碳（CO）会诱导与氧化还原平衡失调相关的代谢变化，这可通过乙酸盐和乳酸盐等有机酸积累的增加得到证明。这表明该生物体通过表达几种替代途径得以存活，包括丙酮酸转化为乳酸，这可平衡还原当量（将NADH氧化为NAD），以及乙酰辅酶A转化为乙酸盐，这可直接产生能量，而此时CO正在结合末端氧化酶。数据还清楚地显示，该生物体在WGS反应过程中获取了能量并得以生长。综合来看，这些数据为进一步开发该生物体的生物技术潜力提供了至关重要的信息。
