Figura Monika, Sitkiewicz Ewa, Świderska Bianka, Milanowski Łukasz, Szlufik Stanisław, Koziorowski Dariusz, Friedman Andrzej
Department of Neurology, Faculty of Health Sciences, Medical University of Warsaw, 03-242 Warsaw, Poland.
Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics Polish Academy of Sciences, 02-106 Warsaw, Poland.
Brain Sci. 2021 May 18;11(5):661. doi: 10.3390/brainsci11050661.
Parkinson's disease (PD) is a progressive neurodegenerative disorder. It affects many organs. Lewy bodies-a histopathological "hallmark" of PD-are detected in about 75% of PD submandibular gland samples. We hypothesize that saliva can be a source of biomarkers of PD. The aim of the study was to evaluate and compare the salivary proteome of PD patients and healthy controls (HC). Salivary samples from 39 subjects (24 PD patients, mean age 61.6 ± 8.2; 15 HC, mean age 60.9 ± 6.7) were collected. Saliva was collected using RNA-Pro-Sal kits. Label-free LC-MS/MS mass spectrometry was performed to characterize the proteome of the saliva. IPA analysis of upstream inhibitors was performed. A total of 530 proteins and peptides were identified. We observed lower concentrations of S100-A16, ARP2/3, and VPS4B in PD group when compared to HC. We conclude that the salivary proteome composition of PD patients is different than that of healthy controls. We observed a lower concentration of proteins involved in inflammatory processes, exosome formation, and adipose tissue formation. The variability of expression of proteins between the two groups needs to be considered.
帕金森病（PD）是一种进行性神经退行性疾病。它会影响多个器官。路易小体——帕金森病的一种组织病理学“标志”——在约75%的帕金森病下颌下腺样本中被检测到。我们假设唾液可能是帕金森病生物标志物的一个来源。本研究的目的是评估和比较帕金森病患者与健康对照（HC）的唾液蛋白质组。收集了39名受试者（24名帕金森病患者，平均年龄61.6±8.2岁；15名健康对照，平均年龄60.9±6.7岁）的唾液样本。使用RNA-Pro-Sal试剂盒收集唾液。采用无标记液相色谱-串联质谱（LC-MS/MS）对唾液蛋白质组进行表征。对上游抑制剂进行了IPA分析。共鉴定出530种蛋白质和肽。与健康对照相比，我们观察到帕金森病组中S100-A16、ARP2/3和VPS4B的浓度较低。我们得出结论，帕金森病患者的唾液蛋白质组组成与健康对照不同。我们观察到参与炎症过程、外泌体形成和脂肪组织形成的蛋白质浓度较低。需要考虑两组之间蛋白质表达的变异性。
