Spyridopoulou Katerina, Aravidou Tamara, Lampri Evangeli, Effraimidou Eleni, Pappa Aglaia, Chlichlia Katerina
Department of Molecular Biology and Genetics, Democritus University of Thrace, University Campus-Dragana, 68100 Alexandroupolis, Greece.
Department of Medicine, Democritus University of Thrace, University Campus-Dragana, 68100 Alexandroupolis, Greece.
Antioxidants (Basel). 2021 May 30;10(6):875. doi: 10.3390/antiox10060875.
is a flowering plant cultivated for its lemon-scented leaves and used in folk medicine for the preparation of tea for the alleviation of symptoms of gastrointestinal disorders, cold, and asthma. The oil extracted from the plant leaves was shown to possess antioxidant potential and to exert antiproliferative activity against breast cancer. The aim of this study was to further investigate potential antitumor effects of oil (LCO) on breast cancer. The in vitro antiproliferative activity of LCO was examined against murine DA3 breast cancer cells by the sulforhodamine B assay. We further explored the LCO's pro-apoptotic potential with the Annexin-PI method. The LCO's anti-migratory effect was assessed by the wound-healing assay. LCO was found to inhibit the growth of DA3 cells in vitro, attenuate their migration, and induce apoptosis. Finally, oral administration of LCO for 14 days in mice inhibited by 55% the size of developing tumors in the DA3 murine tumor model. Noteworthy, in the tumor tissue of LCO-treated mice the apoptotic marker cleaved caspase-3 was elevated, while a reduced protein expression of survivin was observed. These results indicate that LCO, as a source of bioactive compounds, has a very interesting nutraceutical potential.
是一种因其柠檬香味的叶子而被种植的开花植物，在民间医学中用于制备茶，以缓解胃肠道疾病、感冒和哮喘的症状。从植物叶子中提取的油显示具有抗氧化潜力，并对乳腺癌具有抗增殖活性。本研究的目的是进一步研究油（LCO）对乳腺癌的潜在抗肿瘤作用。通过磺酰罗丹明B测定法检测LCO对小鼠DA3乳腺癌细胞的体外抗增殖活性。我们用膜联蛋白 - PI法进一步探索了LCO的促凋亡潜力。通过伤口愈合试验评估LCO的抗迁移作用。发现LCO在体外抑制DA3细胞的生长，减弱其迁移，并诱导凋亡。最后，在小鼠中口服LCO 14天，在DA3小鼠肿瘤模型中，正在发育的肿瘤大小被抑制了55%。值得注意的是，在LCO处理的小鼠的肿瘤组织中，凋亡标志物裂解的半胱天冬酶 - 3升高，而存活素的蛋白表达降低。这些结果表明，LCO作为生物活性化合物的来源，具有非常有趣的营养保健潜力。
