Tiptiri-Kourpeti Angeliki, Fitsiou Eleni, Spyridopoulou Katerina, Vasileiadis Stavros, Iliopoulos Christos, Galanis Alex, Vekiari Stavroula, Pappa Aglaia, Chlichlia Katerina
Department of Molecular Biology and Genetics, Democritus University of Thrace, University Campus, Dragana, 68100 Alexandroupolis, Greece.
ELGO-DEMETER, Institute of Technology of Agricultural Products, 14123 Lykovrisi, Athens, Greece.
Antioxidants (Basel). 2019 Sep 5;8(9):377. doi: 10.3390/antiox8090377.
L. (Cornelian cherry) is a flowering plant indigenous to Europe and parts of Asia, mostly studied for the antimicrobial activity of its juice. In this report, we investigated the composition and the in vitro antioxidant capacity of L. fruit juice from Greece, as well as its antiproliferative properties in vitro and in vivo. The fruits showed a high content of citric, malic, and succinic acid, in contrast to their juice, which had a low concentration of organic acids. The juice demonstrated significant antioxidant activity against the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and modest antiproliferative potential against four human cancer cells lines and one murine: mammary adenocarcinoma MCF-7, hepatocellular carcinoma HepG2 and colon adenocarcinomas Caco2, HT-29, as well as murine colon carcinoma CT26. Cell viability was reduced by 40-50% following incubation of the cells with the highest concentration of the juice. Although Cornelian cherry juice exhibited in vitro growth inhibitory effects against colon carcinoma cells, no tumor growth inhibition was observed in an in vivo experimental colon carcinoma model in mice following prophylactic oral administration of a daily dose of 100 L juice for a period of 10 days. Thus, our findings raise interesting questions for further research on L. fruit juice, and in parallel, the strong antioxidant potential implies that the plant could be further explored and exploited for its protective effect against oxidative damage.
欧洲酸樱桃是一种原产于欧洲和亚洲部分地区的开花植物，主要因其果汁的抗菌活性而受到研究。在本报告中，我们研究了希腊产欧洲酸樱桃果汁的成分、体外抗氧化能力以及其体外和体内的抗增殖特性。与果实相比，其果汁中柠檬酸、苹果酸和琥珀酸含量较高，而有机酸浓度较低。该果汁对自由基2,2-二苯基-1-苦基肼（DPPH）表现出显著的抗氧化活性，对四种人类癌细胞系和一种小鼠癌细胞系具有适度的抗增殖潜力：乳腺腺癌MCF-7、肝癌HepG2、结肠腺癌Caco2、HT-29以及小鼠结肠癌CT26。用最高浓度的果汁孵育细胞后，细胞活力降低了40-50%。尽管欧洲酸樱桃汁在体外对结肠癌细胞具有生长抑制作用，但在小鼠体内实验性结肠癌模型中，连续10天每天口服100μL果汁进行预防性给药后，未观察到肿瘤生长受到抑制。因此，我们的研究结果为进一步研究欧洲酸樱桃汁提出了有趣的问题，同时，其强大的抗氧化潜力意味着该植物可因其对氧化损伤的保护作用而得到进一步探索和利用。
