Hao Ke-Xin, Hao Yun-Fang, Zhang Jie, Xu Xi-Lin, Jiang Jian-Guo
College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China.
Jiangmen Key Laboratory of Traditional Chinese Medicine Ingredients and Their Mechanisms of Action, Guangdong Jiangmen Chinese Medicine College, Jiangmen 529000, China.
Foods. 2024 Jun 29;13(13):2074. doi: 10.3390/foods13132074.
This study was designed to compare the antioxidant, antitumor and anti-inflammatory effects of essential oils from the bark and flower of Rehd. et Wils. Distillation extraction and steam distillation were used to extract EOs from the bark and flower. The results showed that the contents of EOs of SDE-F and SDE-B were much higher than that of SD-F and SD-B. EOs from the bark were rich in eudesmol (especially α-eudesmol) and exhibited a stronger antioxidant effect than the flower. The anti-tumor effects of SD-B and SD-F on HepG2 and MDA-MB-231 cells were better than that of SDE-B and SDE-F. The inhibitory rates of SD-B and SD-F on MDA-MB-231 cells were 59.21% and 48.27%, exceeding that of positive control 5-fluorouracil (47.04%) at 50 μg/mL. All four EOs exhibited excellent anti-inflammatory activities through the regulation of nitric oxide production and pro-inflammation cytokines in LPS-induced RAW 264.7 cells and they also remarkably suppressed the mRNA expressions of nitric oxide synthase, IL-6 and TNF-α at the concentration higher than that of positive control dexamethasone. These results indicated significant differences in the composition, and anti-inflammatory and anti-tumor activities of EOs extracted by different methods and provided a theoretical basis for their development and utilization.
本研究旨在比较川滇绣线菊树皮和花朵精油的抗氧化、抗肿瘤及抗炎作用。采用同时蒸馏萃取法（SDE）和水蒸气蒸馏法（SD）从树皮和花朵中提取精油。结果表明，SDE法提取的花朵精油（SDE-F）和树皮精油（SDE-B）的含量远高于SD法提取的花朵精油（SD-F）和树皮精油（SD-B）。树皮精油富含桉叶醇（尤其是α-桉叶醇），其抗氧化作用强于花朵精油。SD法提取的树皮精油（SD-B）和花朵精油（SD-F）对肝癌细胞HepG2和乳腺癌细胞MDA-MB-231的抗肿瘤效果优于SDE法提取的树皮精油（SDE-B）和花朵精油（SDE-F）。SD-B和SD-F对MDA-MB-231细胞的抑制率分别为59.21%和48.27%，在50 μg/mL时超过阳性对照5-氟尿嘧啶（47.04%）。四种精油均通过调节脂多糖（LPS）诱导的RAW 264.7细胞中一氧化氮的产生和促炎细胞因子，表现出优异的抗炎活性，并且在高于阳性对照地塞米松的浓度下，它们还显著抑制一氧化氮合酶、白细胞介素-6和肿瘤坏死因子-α的mRNA表达。这些结果表明，不同方法提取的精油在成分、抗炎和抗肿瘤活性方面存在显著差异，为其开发利用提供了理论依据。
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