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SCA培养基:一种用于分离所有进化枝的新型培养基。

SCA Medium: A New Culture Medium for the Isolation of All Clades.

作者信息

Ibrahim Ahmad, Peyclit Lucie, Abdallah Rim, Khelaifia Saber, Chamieh Amanda, Rolain Jean-Marc, Bittar Fadi

机构信息

Aix Marseille University, IRD, APHM, MEPHI, 13005 Marseille, France.

IHU-Méditerranée Infection, 13005 Marseille, France.

出版信息

J Fungi (Basel). 2021 May 29;7(6):433. doi: 10.3390/jof7060433.

DOI:10.3390/jof7060433
PMID:34072592
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8226795/
Abstract

is an emerging multidrug-resistant yeast causing nosocomial infections and associated with high mortality in immunocompromised patients. Rapid identification and characterisation are necessary for diagnosis and containing its spread. In this study, we present a selective culture medium for all clades. This medium is sensitive with a limit of detection ranging between 10 and 10 CFU/mL. The 100% specificity of SCA (specific ) medium is confirmed on a set of 135 strains, 50 bacterial species and 200 human stool samples. Thus, this medium specifically selects for isolation from clinical samples, allowing the latter to study its phenotypic profile.

摘要

是一种新兴的多重耐药酵母,可引起医院感染,并与免疫功能低下患者的高死亡率相关。快速鉴定和表征对于诊断和控制其传播至关重要。在本研究中,我们提出了一种针对所有进化枝的选择性培养基。该培养基敏感,检测限在10至10 CFU/mL之间。SCA(特异性)培养基在一组135株菌株、50种细菌和200份人类粪便样本上证实了100%的特异性。因此,这种培养基专门用于从临床样本中分离,从而能够研究其表型特征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/344d/8226795/9015ef9c390f/jof-07-00433-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/344d/8226795/9015ef9c390f/jof-07-00433-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/344d/8226795/9015ef9c390f/jof-07-00433-g001.jpg

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