Amghar Ilham, Ibriz Mohammed, Ibrahimi Maha, Boudra Abdelaali, Gaboun Fatima, Meziani Reda, Iraqi Driss, Mazri Mouaad Amine, Diria Ghizlane, Abdelwahd Rabha
UR Biotechnologie, CRRA-Rabat, Institut National de la Recherche Agronomique, BP 6570, Rabat 10101, Morocco.
Laboratoire de Génétique et Biométrie, Département de Biologie, Faculté des Sciences de Kénitra, Université Ibn Tofail, BP 133, Kenitra 14000, Morocco.
Plants (Basel). 2021 May 26;10(6):1062. doi: 10.3390/plants10061062.
(L.) Skeels is an endangered plant species endemic to Morocco. In recent years, attempts to develop in vitro regeneration systems for this species were made. However, rooting and acclimatization of in vitro plants have been a bottleneck for successful propagation. In the present study, the effects of different concentrations of auxins, putrescine, silver nitrate (AgNO) and ammonium nitrate on the in vitro rooting of adventitious shoots of two argan genotypes "Mejji" and "R'zwa", were evaluated. The highest rooting percentages (86.6% in "Mejji" and 84.4% in "R'zwa") were observed on Murashige and Skoog (MS) medium modified by reducing the ammonium nitrate concentration and supplemented with 1.5 mg L indole-3-butyric acid (IBA), 0.5 mg L 1-naphthalene acetic acid (NAA), 2 mg L AgNO and 160 mg L putrescine. This medium resulted in the development of a good root system after only 10 days of culture. Plantlet acclimatization was carried out using different substrate mixtures, and high survival rates (100%) were observed when the substrate contained either peat alone or a sand-peat mixture (1:1, /). The high percentages of rooting and acclimatization reported in the present study are of high importance for rapid and large-scale propagation of this endangered species.
斯凯尔斯（L.）是摩洛哥特有的一种濒危植物物种。近年来，人们尝试为该物种开发离体再生系统。然而，离体植株的生根和驯化一直是成功繁殖的瓶颈。在本研究中，评估了不同浓度的生长素、腐胺、硝酸银（AgNO）和硝酸铵对两种阿甘基因型“梅吉”和“R'zwa”不定芽离体生根的影响。在通过降低硝酸铵浓度改良的Murashige和Skoog（MS）培养基上，添加1.5毫克/升吲哚-3-丁酸（IBA）、0.5毫克/升1-萘乙酸（NAA）、2毫克/升AgNO和160毫克/升腐胺时，观察到最高的生根率（“梅吉”为86.6%，“R'zwa”为84.4%）。仅培养10天后，这种培养基就使根系发育良好。使用不同的基质混合物对试管苗进行驯化，当基质仅含泥炭或泥炭与沙子的混合物（1:1，体积比）时，观察到高成活率（100%）。本研究报告的高生根率和驯化率对于这种濒危物种的快速大规模繁殖非常重要。
