Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, East China Normal University, Dongchuan Road 500, Shanghai, 200241, China.
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, East China Normal University, Dongchuan Road 500, Shanghai, 200241, China.
Talanta. 2021 Sep 1;232:122434. doi: 10.1016/j.talanta.2021.122434. Epub 2021 Apr 18.
Sialic acid (SA) is a crucial component of glycoproteins and glycolipids on the cellular membrane, which is essential for maintaining the function of cell membranes, such as cell recognition and communication. Simultaneously, sialic acid plays a significant role in many physiological and pathological processes. Hence, it is urgent to develop a simple and sensitive strategy for determining sialic acid. In this work, a new metal-organic framework called UiO-66-NH@B(OH) has been designed and synthesized for the recognition and detection of sialic acid. The boronic acid functional group in UiO-66-NH@B(OH) can bind to a diol moiety of the glycerol side chain of sialic acid, which will attenuate or even quench the fluorescence of UiO-66-NH@B(OH), thus opening a new road to detect sialic acid. Based on the measurement results, sialic acid can be quantitatively measured in a linear range of 0.05-2.5 mmoL/L with the UiO-66-NH@B(OH) probe. The detection limit of sialic acid is as low as 0.025 mmol/L. Furthermore, the boronic-acid functionalized probe UiO-66-NH@B(OH) displays high sensitivity and high selectivity to recognize the sialic acid in mouse serum samples. Therefore, the developed UiO-66-NH@B(OH) can be used as a promising probe to identify and detect sialic acid in the practical application.
唾液酸（SA）是细胞膜上糖蛋白和糖脂的重要组成部分，对于维持细胞膜的功能（如细胞识别和通讯）至关重要。同时，唾液酸在许多生理和病理过程中也起着重要作用。因此，迫切需要开发一种简单灵敏的方法来测定唾液酸。在这项工作中，设计并合成了一种新型的金属有机骨架UiO-66-NH@B(OH)，用于识别和检测唾液酸。UiO-66-NH@B(OH)中的硼酸官能团可以与唾液酸甘油侧链的二醇部分结合，从而削弱甚至猝灭 UiO-66-NH@B(OH)的荧光，从而为检测唾液酸开辟了新途径。基于测量结果，UiO-66-NH@B(OH)探针可在 0.05-2.5 mmoL/L 的线性范围内定量检测唾液酸。唾液酸的检测限低至 0.025 mmol/L。此外，硼酸功能化探针 UiO-66-NH@B(OH)对识别小鼠血清样品中的唾液酸具有高灵敏度和高选择性。因此，开发的 UiO-66-NH@B(OH)可以用作识别和检测实际应用中唾液酸的有前途的探针。
