Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.
The College of Life Science, Northwest University, Xi'an, 710069, China.
Aesthetic Plast Surg. 2024 Feb;48(3):501-509. doi: 10.1007/s00266-023-03783-4. Epub 2024 Jan 10.
Autologous adipose tissue often experiences ischemia and hypoxia after transplantation, leading to low retention rates and unstable operative impacts due to necrotic absorption. Platelet-rich plasma (PRP) can enhance fat regeneration and increase the fat retention rate after transplantation. However, the quick release of growth factors (GFs) in PRP decreases therapeutic efficiency. This study aimed to achieve a slow release of PRP to promote fat retention.
We prepared a dual-network hydrogel (DN gel) based on FDA-approved PRP and sodium alginate (SA) through a simple "one-step" activation process. In vivo study, adipose tissue with saline (control group), SA gel (SA gel group), PRP gel (PRP gel group), and DN gel (DN gel group) was injected subcutaneously into the dorsum of nude mice. At 4 and 12 weeks after injection, tissues were assessed for volume and weight. Hematoxylin and eosin staining (HE) and immunofluorescence staining were performed for histological assessment.
DN gel exhibits long-lasting growth factor effects, surpassing conventional clinical PRP gel regarding vascularization potential. In fat transplantation experiments, DN gel demonstrated improved vascularization of transplanted fat and increased retention rates, showing promise for clinical applications.
DN gel-assisted lipofilling can significantly improve the retention rate and quality of transplanted fat. DN gel-assisted lipofilling, which is considered convenient, is a promising technique to improve neovascularization and fat survival.
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自体脂肪组织移植后常经历缺血缺氧，导致因坏死吸收而留存率低、手术效果不稳定。富血小板血浆（PRP）可增强脂肪再生，增加移植后的脂肪留存率。但 PRP 中生长因子（GFs）的快速释放会降低治疗效率。本研究旨在实现 PRP 的缓慢释放，以促进脂肪留存。
我们通过简单的“一步”激活过程，基于 FDA 批准的 PRP 和海藻酸钠（SA）制备了双网络水凝胶（DN 凝胶）。体内研究中，将生理盐水（对照组）、SA 凝胶（SA 凝胶组）、PRP 凝胶（PRP 凝胶组）和 DN 凝胶（DN 凝胶组）的脂肪组织注射到裸鼠背部皮下。注射后 4 周和 12 周时，评估组织体积和重量。进行苏木精和伊红染色（HE）和免疫荧光染色进行组织学评估。
DN 凝胶表现出持久的生长因子作用，在血管生成潜力方面优于传统临床 PRP 凝胶。在脂肪移植实验中，DN 凝胶显示出改善了移植脂肪的血管化和增加了留存率，具有临床应用的潜力。
DN 凝胶辅助脂肪填充可显著提高移植脂肪的留存率和质量。DN 凝胶辅助脂肪填充被认为是一种方便的技术，可以改善新生血管化和脂肪存活。
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