Dati F, Becker U, Hissung A, Keller F
Research Laboratories of Behringwerke AG, Marburg, Federal Republic of Germany.
Ann Biol Clin (Paris). 1988;46(3):201-10.
The classical coagulation analyses are performed either by using manual methods or by means of various instruments with a different degree of automation. The introduction of chromogenic peptide substrates which can be split by thrombin has led to the development of photometric assays for PT and aPTT determination independent from the fibrinogen concentration and from its conversion to fibrin. After that, turbidimetric methods for the determination of fibrinogen, thrombin time and batroxobin time have been set up allowing the use of photometry for the determination of the most important hemostaseological parameters. For such purposes a new analytical system (ChromoTimeSystem, Behringwerke AG, Marburg/FRG) based on a special instrument and reagents for chromogenic and turbidimetric methods for coagulation and fibrinolysis has been developed. The ChromoTimeSystem allows to perform by photometry all important tests for coagulation and fibrinolysis. The analytical characteristics of this new system are presented.
经典凝血分析可通过手工方法或借助不同自动化程度的各种仪器来进行。可被凝血酶裂解的生色肽底物的引入，使得能够独立于纤维蛋白原浓度及其向纤维蛋白的转化来开发用于PT和活化部分凝血活酶时间（aPTT）测定的光度法。此后，已建立了用于测定纤维蛋白原、凝血酶时间和巴曲酶时间的比浊法，从而能够使用光度法来测定最重要的止血参数。为此目的，基于一种特殊仪器以及用于凝血和纤维蛋白溶解的生色法和比浊法的试剂，开发了一种新的分析系统（ChromoTimeSystem，德国马尔堡贝林werke股份公司）。ChromoTimeSystem允许通过光度法进行所有重要的凝血和纤维蛋白溶解测试。本文介绍了该新系统的分析特性。
