Avci Gulcan, Ulutas Elmas, Ozdemir Vural, Kivrak Ibrahim, Bulbul Aziz
Department of Biochemistry, Faculty of Veterinary Medicine, University of Afyon Kocatepe, Afyonkarahisar, Turkey.
Department of Physiology, Faculty of Veterinary Medicine, University of Bozok, Yozgat, Turkey.
J Food Biochem. 2022 Apr;46(4):e13801. doi: 10.1111/jfbc.13801. Epub 2021 Jun 3.
In our study, the effect of essential oil obtained from Nigella sativa L. (NSE) on thyroid hormones and antioxidant balance in hypothyroidism (HT) and hyperthyroidism (HP) models induced by propylthiouracil(PTU) and L-thyroxine(LT ), respectively, in rats were investigated for 4 weeks. NSE was administered by gastric gavage at a dose of 200 mg/kg body weight. In this study, 48 male Wistar albino rats with an average weight of 180-290 g and age 5-6 months were divided into eight groups, as follows: groups with HT, (1) control, (2) HT, (3) NSE, and (4) HT + NSE; groups with HP, (1) control, (2) HP, (3), and NSE (4) HP + NSE. As a result, we found that NSE administration increased total triiodothyronine (TT ) and decreased nitric oxide in HT + NSE. Besides, it decreased TT in HP + NSE and increased total antioxidant capacity. Our findings suggest that NSE may have beneficial effects on thyroid gland abnormalities owing to its antioxidant properties. PRACTICAL APPLICATIONS: Essential oils derived from Nigella sativa L. seed contain many bioactive substances such as thymoquinone and cymene. This paper emphasizes the effect of NSE on thyroid hormone abnormalities and negative oxidative state that occurs in HT and HP models. The present study provides evidence of a positive effect of NSE particularly on TT levels in the HT and HP models. It can therefore be assumed that NSE could be used as a supportive natural alternative source to improve thyroid hormone levels and relieve increased oxidative stress.
在我们的研究中，分别对用丙硫氧嘧啶（PTU）和L-甲状腺素（LT）诱导的甲状腺功能减退（HT）和甲状腺功能亢进（HP）大鼠模型，研究了黑种草籽精油（NSE）对甲状腺激素和抗氧化平衡的影响，为期4周。通过胃管以200mg/kg体重的剂量给予NSE。在本研究中，将48只平均体重为180 - 290g、年龄为5 - 6个月的雄性Wistar白化大鼠分为八组，如下：HT组，（1）对照组，（2）HT组，（3）NSE组，（4）HT + NSE组；HP组，（1）对照组，（2）HP组，（3）NSE组，（4）HP + NSE组。结果，我们发现给予NSE可使HT + NSE组的总三碘甲状腺原氨酸（TT）升高，一氧化氮降低。此外，它可使HP + NSE组的TT降低，并提高总抗氧化能力。我们的研究结果表明，NSE因其抗氧化特性可能对甲状腺异常具有有益作用。实际应用：黑种草籽精油含有许多生物活性物质，如百里醌和对异丙基甲苯。本文强调了NSE对HT和HP模型中出现的甲状腺激素异常和负氧化状态的影响。本研究提供了证据，证明NSE对HT和HP模型中的TT水平具有积极作用。因此，可以假设NSE可作为一种支持性的天然替代来源，以改善甲状腺激素水平并减轻氧化应激增加的情况。
