伊朗北部E-钙黏蛋白基因-160C>A和-347GA>G多态性与胃癌的相关性
Correlation of -160C > A and -347GA > G polymorphisms in E-cadherin gene and gastric cancer in north of Iran.
作者信息
Shekarriz Ramin, Alikhani Reza, Ghasemi Mohaddeseh, Navaei Reza Alizadeh, Hashemi-Soteh Mohammad Bagher
机构信息
Department of Hematology and Oncology, Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
出版信息
J Res Med Sci. 2021 Jan 28;26:3. doi: 10.4103/jrms.JRMS_50_20. eCollection 2021.
BACKGROUND
E-cadherin ( gene) is a protein involved in cell-cell adhesion. There are reports on the association of -160C > A (rs16260) and -347GA > G (rs5030625) polymorphisms in the 5'-promoter region of the gene with tumor development and progression of gastric cancer. This study aimed to examine the potential relationship between these two polymorphisms and gastric cancer in patients from Mazandaran province, Northern Iran.
MATERIALS AND METHODS
A case-control study was conducted to test 97 patients and 95 healthy controls. Genomic DNA was extracted from peripheral blood followed by polymerase chain reaction amplification. Genotyping analysis was carried out using restriction fragment length polymorphism analysis for two potentially functional polymorphisms.
RESULTS
Heterozygous genotype GA/G versus GA/GA of rs5030625 (-347 GA > G) was found to be associated with increased risk of gastric cancer in the people studied (odds ratio = 5.73, 95% confidence interval = 2.11-15.56, = 0.001). Furthermore, AA or CA genotype in -160C > A polymorphism did not show any increased risk of gastric cancer ( = 0.559).
CONCLUSION
The present study revealed that GA/G genotype of rs5030625 (-347 GA > G) polymorphism is associated with gastric cancer in Northern Iran.
背景
E-钙黏蛋白（基因）是一种参与细胞间黏附的蛋白质。有报道称该基因5'-启动子区域的-160C＞A（rs16260）和-347GA＞G（rs5030625）多态性与胃癌的肿瘤发生和进展有关。本研究旨在探讨伊朗北部马赞德兰省患者中这两种多态性与胃癌之间的潜在关系。
材料与方法
进行了一项病例对照研究，对97例患者和95例健康对照进行检测。从外周血中提取基因组DNA，随后进行聚合酶链反应扩增。使用限制性片段长度多态性分析对两个潜在的功能性多态性进行基因分型分析。
结果
发现rs5030625（-347GA＞G）的杂合基因型GA/G与GA/GA相比，在所研究人群中与胃癌风险增加相关（比值比=5.73，95%置信区间=2.11-15.56，P=0.001）。此外，-160C＞A多态性中的AA或CA基因型未显示出任何胃癌风险增加（P=0.559）。
结论
本研究表明，rs5030625（-347GA＞G）多态性的GA/G基因型与伊朗北部的胃癌有关。
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