Raja Daim Asif, Munir Fazeelah, Shah Muhammad Raza, Bhanger Muhammad Iqbal, Malik Muhammad Imran
H.E.J. Research Institute of Chemistry, University of Karachi, Karachi 75270, Pakistan.
Third World Center for Science and Technology, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi 75270, Pakistan.
R Soc Open Sci. 2021 May 26;8(5):210185. doi: 10.1098/rsos.210185.
The development of metal nanoparticle-based facile colorimetric assays for drugs and insecticides is an emerging area of current scientific research. In the present work, polypropylene glycol was used for stabilization of gold nanoparticles (AuNPs) in a simple one-pot two-phase process and subsequently employed it for the specific detection of cephradine (CPH). The characterization of the prepared PPG-AuNPs was conducted through various analytical techniques such as UV-visible spectrophotometry, Fourier transform infrared spectroscopy, atomic force microscopy (AFM), zeta potential and zetasizer techniques. As the major target of the study, the stabilized PPG-AuNPs were employed for colorimetric detection of CPH and other drugs. Typical wine-red colour of PPG-AuNPs disappeared immediately and surface plasmon resonance band quenched by addition of CPH in the presence of several other interferents (drugs and salts) and in real samples. PPG-AuNPs permitted efficient, selective, reliable and rapid determination in a concentration range of 0.01-120 mM with a detection limit (LoD) of 11.0 mM. The developed sensor has the potential to be used for fast scanning of pharmaceutical formulations for quantification of CPH at production facilities.
基于金属纳米颗粒的药物和杀虫剂简易比色测定法的开发是当前科学研究的一个新兴领域。在本工作中，聚丙二醇用于在简单的一锅两相过程中稳定金纳米颗粒（AuNPs），随后将其用于头孢拉定（CPH）的特异性检测。通过紫外可见分光光度法、傅里叶变换红外光谱法、原子力显微镜（AFM）、zeta电位和zetasizer技术等各种分析技术对制备的PPG-AuNPs进行了表征。作为研究的主要目标，稳定化的PPG-AuNPs用于CPH和其他药物的比色检测。在存在其他几种干扰物（药物和盐）以及实际样品的情况下，加入CPH后，PPG-AuNPs典型的酒红色立即消失，表面等离子体共振带猝灭。PPG-AuNPs能够在0.01-120 mM的浓度范围内进行高效、选择性、可靠和快速的测定，检测限（LoD）为11.0 mM。所开发的传感器有潜力用于在生产设施中对药物制剂进行快速扫描以定量CPH。
