H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan.
Third World Center for Science and Technology, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi, 75270, Pakistan.
Mikrochim Acta. 2024 Feb 28;191(3):164. doi: 10.1007/s00604-024-06237-z.
A reliable, rapid, and inexpensive nano-sized chemosensor is presented for methamidophos (MET) - an insecticide. Poly(lactic acid) (PLA)-stabilized gold nanoparticles (AuNPs) were synthesized by a simple one-pot, two-phase chemical reduction method. The synthesized PLA-AuNPs were subsequently employed for selective, efficient, and quantitative detection of MET. MET is one of the highly toxic pesticides used for eradication of agricultural and urban insects. Upon the addition of MET, the wine-red color of PLA-AuNPs swiftly transformed into greyish-blue, further corroborated by a significant bathochromic and hyperchromic shift in the SPR band. The presence of other interfering insecticides, metal salts, and drugs did not have any pronounced effect on quantitative MET detection. The detection limit, the quantification limit, and linear dynamic range of MET utilizing PLA-AuNPs were 0.0027 µM, 0.005 µM, and 0.005-1000 µM, respectively. The PLA-AuNP-based assay renders an efficient, rapid, accurate, and selective quantification of MET in food, biological, and environmental samples. The proposed sensor provides an appropriate platform for fast and on-the-spot determination of MET without requiring a well-equipped lab setup.
本文提出了一种可靠、快速且廉价的纳米尺寸化学传感器，用于检测甲胺磷（MET）——一种杀虫剂。通过简单的一锅两相反相化学还原法合成了聚乳酸（PLA）稳定的金纳米粒子（AuNPs）。随后，将合成的 PLA-AuNPs 用于 MET 的选择性、高效和定量检测。MET 是用于消灭农业和城市昆虫的高毒性农药之一。加入 MET 后，PLA-AuNPs 的酒红色迅速转变为灰蓝色，SPR 带的明显红移和增色进一步证实了这一点。其他干扰性杀虫剂、金属盐和药物的存在对定量 MET 检测没有任何明显影响。利用 PLA-AuNPs 检测 MET 的检测限、定量限和线性动态范围分别为 0.0027 µM、0.005 µM 和 0.005-1000 µM。基于 PLA-AuNP 的测定方法可在食品、生物和环境样品中对 MET 进行高效、快速、准确和选择性的定量检测。该传感器提供了一个合适的平台，无需配备齐全的实验室设备即可快速进行现场测定 MET。
