Porter School of Environment and Earth Sciences, Tel Aviv University, Tel Aviv, Israel.
School of Biomedical Engineering, Tel Aviv University, Tel Aviv, Israel.
Sci Rep. 2021 Jun 4;11(1):11843. doi: 10.1038/s41598-021-90903-2.
Extracellular matrix (ECM) provides structural support for cell growth, attachments and proliferation, which greatly impact cell fate. Marine macroalgae species Ulva sp. and Cladophora sp. were selected for their structural variations, porous and fibrous respectively, and evaluated as alternative ECM candidates. Decellularization-recellularization approach was used to fabricate seaweed cellulose-based scaffolds for in-vitro mammalian cell growth. Both scaffolds were confirmed nontoxic to fibroblasts, indicated by high viability for up to 40 days in culture. Each seaweed cellulose structure demonstrated distinct impact on cell behavior and proliferation rates. The Cladophora sp. scaffold promoted elongated cells spreading along its fibers' axis, and a gradual linear cell growth, while the Ulva sp. porous surface, facilitated rapid cell growth in all directions, reaching saturation at week 3. As such, seaweed-cellulose is an environmentally, biocompatible novel biomaterial, with structural variations that hold a great potential for diverse biomedical applications, while promoting aquaculture and ecological agenda.
细胞外基质 (ECM) 为细胞生长、附着和增殖提供结构支持，这对细胞命运有很大影响。选择海藻物种石莼和刚毛藻作为 ECM 候选物，是因其结构上的差异，分别具有多孔和纤维状。使用脱细胞-再细胞化方法来制备基于海藻纤维素的支架，以用于体外哺乳动物细胞生长。两种支架都被证实对成纤维细胞无毒，在培养中高达 40 天的高存活率表明了这一点。每种海藻纤维素结构对细胞行为和增殖率都有明显的影响。刚毛藻支架促进细胞沿着纤维轴的方向伸长和扩散，细胞呈线性生长；而石莼的多孔表面则促进细胞在各个方向上快速生长，在第 3 周达到饱和。因此，海藻纤维素是一种具有环境相容性的新型生物材料，其结构变化为各种生物医学应用提供了巨大的潜力，同时也促进了水产养殖和生态议程。
