College of life Science / Xinjiang Production and Construction Corps Key Laboratory of Oasis Town and Mountain-basin System Ecology, North 4 Street, College of life Science, Shihezi University, Shihezi, 832003, People's Republic of China.
Department of Plant protection, College of Agriculture, Shihezi University, Shihezi, Xinjiang, 832000, China.
BMC Microbiol. 2021 Jun 6;21(1):170. doi: 10.1186/s12866-021-02222-1.
The unique climatic conditions of the Xinjiang region nurture rich melon and fruit resources, the melon and fruit sugar sources provide sufficient nutrients for the survival of yeast, and the diverse habitats accompanied by extreme climatic conditions promote the production of yeast diversity and strain resources. However, the relationship between yeast species and their relationship with environmental factors in the soil of Xinjiang specialty cash crop Hami melon is not clear. Here, we aimed to characterize the diversity, community structure, and relationship between yeast species and environmental factors in Hami melon orchards soils in different regions of Xinjiang, China.
Based on Illumina MiSeq high-throughput sequencing analysis of the D1 domain of the LSU rRNA genes, the community richness of yeast in the soil of Northern Xinjiang was higher than in the Southern and Eastern Xinjiang, but the community diversity was significantly lower in the Northern Xinjiang than in the Southern and Eastern Xinjiang. A total of 86 OTUs were classified into 59 genera and 86 species. Most OTUs (90.4%) belonged to the Basidiomycota; only a few (9.6%) belonged to Ascomycota. The most dominant species in the Southern, Eastern and Northern Xinjiang were Filobasidium magnum (17.90%), Solicoccozyma aeria (35.83%) and Filobasidium magnum (75.36%), respectively. Principal coordinates analysis (PCoA) showed that the yeast community composition in the soils of the three regions were obviously different, with the Southern and Eastern Xinjiang having more similar yeast community. Redundancy analysis (RDA) showed that soil factors such as conductivity (CO), total phosphorus (TP) and Total potassium (TK) and climate factors such as average annual precipitation (PRCP), relative humidity (RH) and net solar radiation intensity (SWGNT) were significantly correlated with yeast communities (P < 0.05).
There are abundant yeast resources in the rhizosphere soil of Hami melon orchard in Xinjiang, and there are obvious differences in the diversity and community structure of yeast in the three regions of Xinjiang. Differences in climatic factors related to precipitation, humidity and solar radiation intensity and soil factors related to conductivity, total phosphorus and total potassium are key factors driving yeast diversity and community structure.
新疆独特的气候条件孕育了丰富的瓜果资源，瓜果糖源为酵母的生存提供了充足的养分，多样的生境伴随着极端的气候条件，促进了酵母多样性和菌株资源的产生。然而，关于新疆特色经济作物哈密瓜土壤中的酵母物种与环境因素之间的关系尚不清楚。在这里，我们旨在描述中国新疆不同地区哈密瓜果园土壤中酵母的多样性、群落结构以及酵母物种与环境因素之间的关系。
基于 Illumina MiSeq 高通量测序对 LSU rRNA 基因 D1 区的分析，北疆地区土壤中酵母的群落丰富度高于南疆和东疆，但北疆地区的群落多样性明显低于南疆和东疆。共分类出 86 个 OTUs，分为 59 属 86 种。大多数 OTUs（90.4%）属于担子菌门；只有少数（9.6%）属于子囊菌门。南疆、东疆和北疆最优势的物种分别是巨孢毕赤酵母（17.90%）、好氧掷孢酵母（35.83%）和巨孢毕赤酵母（75.36%）。主坐标分析（PCoA）表明，三个地区土壤中的酵母群落组成明显不同，南疆和东疆的酵母群落具有更多的相似性。冗余分析（RDA）表明，土壤因素如电导率（CO）、总磷（TP）和总钾（TK）以及气候因素如年平均降水量（PRCP）、相对湿度（RH）和净太阳辐射强度（SWGNT）与酵母群落显著相关（P<0.05）。
新疆哈密瓜果园根际土壤中酵母资源丰富，新疆三个地区的酵母多样性和群落结构存在明显差异。与降水、湿度和太阳辐射强度相关的气候因素以及与电导率、总磷和总钾相关的土壤因素是驱动酵母多样性和群落结构的关键因素。
