Zhang Haihan, Feng Ji, Chen Shengnan, Li Baoqin, Sekar Raju, Zhao Zhenfang, Jia Jingyu, Wang Yue, Kang Pengliang
Key Laboratory of Northwest Water Resources, Environment and Ecology, Ministry of Education, Xi'an University of Architecture and Technology, Xi'an, China.
Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an, China.
Front Microbiol. 2018 Jun 18;9:1291. doi: 10.3389/fmicb.2018.01291. eCollection 2018.
Activated sludge microbial community composition is a key bio-indicator of the sustainability of wastewater treatment systems. Therefore, a thorough understanding of the activated sludge microbial community dynamics is critical for environmental engineers to effectively manage the wastewater treatment plants (WWTPs). However, fungal communities associated with activated sludge have been poorly elucidated. Here, the activated sludge fungal community in 18 geographically distributed WWTPs was determined by using Illumina sequencing. The results showed that differences in activated sludge fungal community composition were observed among all WWTPs and also between oxidation ditch and anaerobic-anoxic-aerobic (A/A/O) systems. Ascomycota was the largest phyla, followed by Basidiomycota in all samples. Sporidiobolales and Pezizales were the most abundant order in oxidation ditch and A/A/O systems, respectively. The network analysis indicated cooperative and co-occurrence interactions between fungal taxa in order to accomplish the wastewater treatment process. sp., sp., sp., sp., sp., and sp., were found to have statistically significant interactions. Redundancy analysis revealed that temperature, total phosphorus, pH, and ammonia nitrogen were significantly affected the fungal community. This study sheds light on providing the ecological characteristics of activated sludge fungal communities and useful guidance for improving wastewater treatment performance efficiency.
活性污泥微生物群落组成是废水处理系统可持续性的关键生物指标。因此，深入了解活性污泥微生物群落动态对于环境工程师有效管理污水处理厂（WWTPs）至关重要。然而，与活性污泥相关的真菌群落尚未得到充分阐明。在此，通过Illumina测序确定了18个地理分布的污水处理厂中的活性污泥真菌群落。结果表明，在所有污水处理厂之间以及氧化沟和厌氧-缺氧-好氧（A/A/O）系统之间均观察到活性污泥真菌群落组成的差异。子囊菌门是所有样本中最大的门类，其次是担子菌门。掷孢酵母目和盘菌目分别是氧化沟和A/A/O系统中最丰富的目。网络分析表明真菌分类群之间存在协同和共现相互作用，以完成废水处理过程。发现sp.、sp.、sp.、sp.、sp.和sp.具有统计学上显著的相互作用。冗余分析表明，温度、总磷、pH值和氨氮对真菌群落有显著影响。本研究为揭示活性污泥真菌群落的生态特征以及提高废水处理性能效率提供了有用的指导。
