Jansen Renate L M, Santana-Molina Carlos, van den Noort Marco, Devos Damien P, van der Klei Ida J
Molecular Cell Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, Netherlands.
Centro Andaluz de Biología del Desarrollo, Consejo Superior de Investigaciones Científicas, Universidad Pablo de Olavide, Seville, Spain.
Front Cell Dev Biol. 2021 May 20;9:654163. doi: 10.3389/fcell.2021.654163. eCollection 2021.
PEX genes encode proteins involved in peroxisome biogenesis and proliferation. Using a comparative genomics approach, we clarify the evolutionary relationships between the 37 known PEX proteins in a representative set of eukaryotes, including all common model organisms, pathogenic unicellular eukaryotes and human. A large number of previously unknown PEX orthologs were identified. We analyzed all PEX proteins, their conservation and domain architecture and defined the core set of PEX proteins that is required to make a peroxisome. The molecular processes in peroxisome biogenesis in different organisms were put into context, showing that peroxisomes are not static organelles in eukaryotic evolution. Organisms that lack peroxisomes still contain a few PEX proteins, which probably play a role in alternative processes. Finally, the relationships between PEX proteins of two large families, the Pex11 and Pex23 families, were analyzed, thereby contributing to the understanding of their complicated and sometimes incorrect nomenclature. We provide an exhaustive overview of this important eukaryotic organelle.
PEX基因编码参与过氧化物酶体生物发生和增殖的蛋白质。我们采用比较基因组学方法，阐明了包括所有常见模式生物、致病性单细胞真核生物和人类在内的一组代表性真核生物中37种已知PEX蛋白之间的进化关系。鉴定出了大量以前未知的PEX直系同源物。我们分析了所有PEX蛋白、它们的保守性和结构域架构，并定义了形成过氧化物酶体所需的PEX蛋白核心集。将不同生物体中过氧化物酶体生物发生的分子过程置于相应背景下，表明过氧化物酶体在真核生物进化中并非静态细胞器。缺乏过氧化物酶体的生物体仍含有一些PEX蛋白，这些蛋白可能在替代过程中发挥作用。最后，分析了两个大家族（Pex11和Pex23家族）的PEX蛋白之间的关系，从而有助于理解它们复杂且有时不正确的命名法。我们对这个重要的真核细胞器进行了详尽概述。
