Xu Ye, Zhang Ying, Zheng Xiangkuo, Yu Kaihang, Sun Yao, Liao Wenli, Jia Huaiyu, Xu Chunquan, Zhou Tieli, Shen Mo
Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
Department of Medical Lab Science, School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
Eur J Clin Microbiol Infect Dis. 2021 Dec;40(12):2651-2656. doi: 10.1007/s10096-021-04287-2. Epub 2021 Jun 7.
Modifying enzyme-CrpP and its variants reduced the MICs of fluoroquinolones in Pseudomonas aeruginosa. This study investigated the dissemination and functional characteristics of CrpP-like in P. aeruginosa from China. The positive rate of crpP-like genes in 228 P. aeruginosa was 25.4% (58/228), and 6 new crpP-like genes were determined. Transformation experiments showed that CrpP-like had a low effect on CIP and LEV susceptibility. The genetic of crpP-positive was diverse. Furthermore, the mean expression level of crpP was no statistical difference between fluoroquinolone-susceptible and -resistant group (P > 0.05). CrpP-like may not play a significant role in fluoroquinolone resistance in P. aeruginosa.
修饰酶-CrpP及其变体降低了铜绿假单胞菌中氟喹诺酮类药物的最低抑菌浓度。本研究调查了中国铜绿假单胞菌中CrpP样蛋白的传播情况及其功能特性。228株铜绿假单胞菌中crpP样基因的阳性率为25.4%（58/228），并确定了6个新的crpP样基因。转化实验表明，CrpP样蛋白对环丙沙星（CIP）和左氧氟沙星（LEV）的敏感性影响较小。crpP阳性菌株的基因具有多样性。此外，crpP的平均表达水平在氟喹诺酮敏感组和耐药组之间无统计学差异（P>0.05）。CrpP样蛋白可能在铜绿假单胞菌对氟喹诺酮的耐药性中不起重要作用。
