Effect of creating a fed-batch like condition using carbon to nitrogen ratios on lipid accumulation in Rhodosporidium toruloides-1588.

Utilizing the undetoxified wood hydrolysate to accumulate maximum lipids in Rhodosporidium toruloides under optimum conditions has been regarded as a renewable and cost-effective strategy. The current investigation aims to identify the best carbon to nitrogen (C/N 20, 70, and 120) ratio for maximum lipid accumulation in R. toruloides-1588 using wood hydrolysate. Additionally, a fed-batch-like condition was employed, where C/N ratios were maintained during the fermentation that inherently decreases in batch fermentation. The C/N ratio 70 has been identified as the best condition with 3 times higher lipid accumulation (43% w/w) than the control. Additionally, >95% and 70% of glucose and xylose consumption were observed, respectively. Moreover, 50% increase in polyunsaturated fatty acids compared to the control media reinforced the potential of R. toruloides-1588 to thrive on undetoxified hydrolysate, high lipid productivity (3.8 mg/g of dry weight per hour) and produce high value monosaturated and polyunsaturated fatty acids.