National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research), NHC Key Laboratory of Parasite and Vector Biology, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Shanghai200025, China.
Department of Parasitology, Zunyi Medical University, Zunyi563000, China.
Parasitology. 2021 Sep;148(11):1360-1365. doi: 10.1017/S0031182021000913. Epub 2021 Jun 8.
Giardia duodenalis is a common zoonotic intestinal pathogen. It has been increasingly reported in humans and animals; however, genotyping information for G. duodenalis in captive animals is still limited. This study was conducted to assess the prevalence and multilocus genotyping of G. duodenalis in captive animals in zoological gardens in Shanghai, China. A total of 678 fresh fecal samples were randomly collected from captive animals including non-human primates (NHPs) (n = 190), herbivores (n = 190), carnivores (n = 151), birds (n = 138) and reptiles (n = 9) in a zoo and were examined for the presence of G. duodenalis using nested polymerase chain reaction (nested PCR). All G. duodenalis positive samples were assayed with PCR followed by sequencing at β-giardin (bg), glutamate dehydrogenase (gdh) and triose phosphate isomerase (tpi) genes. In this study, 42 specimens (6.2%) were tested G. duodenalis-positive of the 678 fecal samples examined based on a single locus. A total of 30 (4.4%), 30 (4.4%) and 22 (3.2%) specimens were successfully amplified and sequenced at gdh, tpi and bg loci, respectively. Assemblages A and B were identified with assemblage B dominating in NHPs. Sequence analysis demonstrated that one, two and five new isolates were identified at bg, gdh and tpi loci. DNA sequences and new assemblage-subtypes of zoonotic G. duodenalis assemblages A and B were identified in the current study. Our data indicate the occurrence and molecular diversity of G. duodenalis and the potential zoonotic transmission in captive animals in China.
十二指肠贾第鞭毛虫是一种常见的动物源性肠道病原体。它在人类和动物中的报道越来越多；然而，圈养动物中十二指肠贾第鞭毛虫的基因分型信息仍然有限。本研究旨在评估中国上海动物园圈养动物中十二指肠贾第鞭毛虫的流行情况和多位点基因分型。从动物园中随机采集了 678 份新鲜粪便样本，包括非人类灵长类动物（NHPs）（n = 190）、食草动物（n = 190）、食肉动物（n = 151）、鸟类（n = 138）和爬行动物（n = 9），采用巢式聚合酶链反应（nested PCR）检测粪便样本中是否存在十二指肠贾第鞭毛虫。所有 G. 十二指肠阳性样本均采用 PCR 进行检测，然后在β-微管蛋白（bg）、谷氨酸脱氢酶（gdh）和磷酸丙糖异构酶（tpi）基因上进行测序。在本研究中，根据单一基因座，对 678 份粪便样本进行检测，有 42 份（6.2%）为 G. 十二指肠阳性。在 gdh、tpi 和 bg 基因座上成功扩增和测序的样本分别有 30 个（4.4%）、30 个（4.4%）和 22 个（3.2%）。鉴定出了 A 组和 B 组，B 组在 NHPs 中占主导地位。序列分析表明，在 bg、gdh 和 tpi 基因座上分别鉴定出 1、2 和 5 个新分离株。本研究鉴定出了具有潜在人兽共患性的动物源性 G. 十二指肠鞭毛虫 A 组和 B 组的 DNA 序列和新的亚型。本研究结果表明，中国圈养动物中存在 G. 十二指肠鞭毛虫的发生和分子多样性，以及潜在的人兽共患传播。
