Programa de Pós-Graduação em Biologia Celular e Molecular, Escola de Ciências da Saúde e da Vida, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Programa de Pós-Graduação em Medicina e Ciências da Saúde, Escola de Medicina, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Toxicol Appl Pharmacol. 2021 Sep 1;426:115616. doi: 10.1016/j.taap.2021.115616. Epub 2021 Jun 6.
Oxytetracycline (OTC) is one of the broad-spectrum antibiotics widely used for the treatment of fish-farm infection. Considering that behavior is directly related to reproduction, individual fitness, and survival, it is important to evaluate the impact of antibiotics on the behavioral repertoire in fish. Zebrafish (Danio rerio) presents a well-described behavioral repertoire to reliably demonstrate complex responses to chemical compound exposure. This work aims to identify the role of OTC in comprehensive behavioral parameters and whole-body cortisol levels in adult zebrafish. Here we report that OTC exposure (10, 20, and 100 mg/L) induces an anxiogenic-like phenotype in the novel tank test. OTC exposure also changes the behavior of social interaction with a shoal of unknown zebrafish - characterized as a stimulus group. Zebrafish exposed to OTC (10 mg/L) remains a longer period in the stimulus zone when compared to the control group. Clonazepam (0.006 mg/L) was able to reverse anxiogenic-like behavior and the changes in social behavior induced by OTC. We also demonstrated that cortisol levels were significantly decreased after exposure to OTC (10, 20, and 100 mg/L), which were not reversed by clonazepam. These findings highlight the growing utility of zebrafish as a model to understand the impact of antibiotics on behavior and their underlying mechanisms.
土霉素（OTC）是一种广谱抗生素，广泛用于治疗水产养殖中的感染。由于行为直接关系到繁殖、个体适应性和生存，因此评估抗生素对鱼类行为表现的影响非常重要。斑马鱼（Danio rerio）具有描述良好的行为表现，可可靠地展示对化学化合物暴露的复杂反应。本研究旨在确定 OTC 在成年斑马鱼的综合行为参数和全身体皮质醇水平中的作用。在这里，我们报告 OTC 暴露（10、20 和 100mg/L）在新鱼缸测试中诱导出焦虑样表型。OTC 暴露还改变了与不熟悉的斑马鱼群体的社交互动行为——表现为刺激群体。与对照组相比，暴露于 OTC（10mg/L）的斑马鱼在刺激区停留的时间更长。氯硝西泮（0.006mg/L）能够逆转 OTC 诱导的焦虑样行为和社交行为变化。我们还表明，暴露于 OTC（10、20 和 100mg/L）后皮质醇水平显著降低，但氯硝西泮不能逆转这种降低。这些发现强调了利用斑马鱼作为模型来理解抗生素对行为及其潜在机制的影响的日益增加的实用性。
