Heidemann M K
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5749-53. doi: 10.1073/pnas.74.12.5749.
Rana pipiens with skin dorosoventrally reversed can respond to stimulation of the back with forelimb wipes to the belly and to stimulation of the belly with hindlimb wipes to the back. These "misdirected wiping responses" have been explained in terms of two alternative hypotheses of nerve regeneration: nerve respecification or selective reinnervation. Experimental behavioral and neurophysiological experiments reported here support the selective reinnervation hypothesis. Severing ventral nerves, which normally innervate the belly, greatly reduced the percentage of misdirected responses on stimulation of belly skin grafted to the back, while severing dorsal nerves, which normally innervate the back, increased the percentage of misdirected responses elicited under the same circumstances. Moreover, neurophysiological recordings of grafted animals showed three effects of skin grafting on nerve distributions: (i) termination of dorsal and ventral nerve receptive field at graft edges; (ii) overlap of nonadjacent ventral nerve receptive fields; and (iii) dorsal coursing of ventral nerves to reinnervate target belly skin displaced to the back. These neurophysiological observations, and particularly the third effect, also support selective reinnervation as the mechanism of nerve regeneration in skin-grafted Rana pipiens.
皮肤背腹颠倒的豹蛙能够对背部的刺激做出反应，用前肢擦拭腹部；对腹部的刺激做出反应，用后肢擦拭背部。这些“错误定向的擦拭反应”已根据神经再生的两种替代假说进行了解释：神经重新指定或选择性再支配。本文报道的实验行为和神经生理学实验支持选择性再支配假说。切断通常支配腹部的腹侧神经，极大地降低了对移植到背部的腹部皮肤进行刺激时错误定向反应的百分比，而切断通常支配背部的背侧神经，则增加了在相同情况下引发的错误定向反应的百分比。此外，移植动物的神经生理学记录显示了皮肤移植对神经分布的三种影响：（i）背侧和腹侧神经感受野在移植边缘终止；（ii）不相邻腹侧神经感受野的重叠；（iii）腹侧神经向背侧走行以重新支配移位到背部的目标腹部皮肤。这些神经生理学观察结果，尤其是第三种影响，也支持选择性再支配作为皮肤移植豹蛙神经再生的机制。
