Shima Felix K, Gberindyer Fidelis A, Tion Matthew T, Fagbohun Olusegun A, Omobowale Temidayo O, Nottidge Helen O
Department of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria.
Department of Veterinary Pharmacology and Toxicology, Federal University of Agriculture, Makurdi, Nigeria.
Top Companion Anim Med. 2021 Nov;45:100551. doi: 10.1016/j.tcam.2021.100551. Epub 2021 Jun 11.
In dogs, canine parvovirus (CPV) enteritis is associated with high morbidity and fatality rates requiring early diagnosis to facilitate treatment and reduce its spread. In recent times, various commercial immunochromatographic (IC) test kits are available for its rapid diagnosis, which require an assessment of their accuracy. Therefore, precision of a point-of-care IC combination test kit for canine coronavirus (CCoV)/CPV faecal antigen detection was evaluated in this study. Multicentred random faecal samples from 115 dogs with gastroenteritis were checked for the presence of CPV antigens using the SensPERT IC combination test kit and the result was compared with polymerase chain reaction (PCR) as a reference test. Parvovirus was detected in 105 (91.3%) and 108 (93.9%) faecal samples by the point-of-care test kit and PCR, respectively. The point-of-care IC test kit showed 95.4% relative sensitivity, 71.4% specificity, 98.1% positive predictive value, 50.0% negative predictive value, and 93.9% accuracy comparable to conventional PCR in the samples tested. This point-of-care test kit also demonstrated a fair positive likelihood ratio (3.34), a very low negative likelihood ratio (0.07) and a moderate agreement (Kappa = 0.6) compared with conventional PCR. This test kit has shown to be very useful in the screening of dogs for CPV infection, and is a reliable alternative for diagnosing CPV both in conventional laboratories and remote areas without laboratories. Negative results in the IC testing with high suspicion of CPV infection should be further confirmed using superior test such as PCR.
在犬类中，犬细小病毒（CPV）肠炎的发病率和死亡率很高，需要早期诊断以促进治疗并减少其传播。近年来，有各种商业免疫层析（IC）检测试剂盒可用于快速诊断，这需要评估其准确性。因此，本研究评估了一种用于检测犬冠状病毒（CCoV）/CPV粪便抗原的即时检测IC组合试剂盒的准确性。使用SensPERT IC组合检测试剂盒对115只患有肠胃炎的犬的多中心随机粪便样本进行CPV抗原检测，并将结果与作为参考检测的聚合酶链反应（PCR）进行比较。即时检测试剂盒和PCR分别在105份（91.3%）和108份（93.9%）粪便样本中检测到细小病毒。在所检测的样本中，即时检测IC试剂盒显示出95.4%的相对灵敏度、71.4%的特异性、98.1%的阳性预测值、50.0%的阴性预测值以及与传统PCR相当的93.9%的准确率。与传统PCR相比，该即时检测试剂盒还显示出较好的阳性似然比（3.34）、非常低的阴性似然比（0.07）和中等一致性（Kappa = 0.6）。该检测试剂盒已证明在筛查犬类CPV感染方面非常有用，并且是在传统实验室和无实验室的偏远地区诊断CPV的可靠替代方法。对于高度怀疑CPV感染但IC检测结果为阴性的情况，应使用如PCR等更高级的检测方法进一步确认。
