Fynn Biotechnologies Ltd., Mills Institute for Personalized Cancer Care, Jinan City, Shandong Province, P. R. China.
Biotechnol J. 2021 Sep;16(9):e2100041. doi: 10.1002/biot.202100041. Epub 2021 Jun 27.
The tumor microenvironment (TME) harbors heterogeneous contents and plays critical roles in tumorigenesis, metastasis, and drug resistance. Therefore, the deconvolution of the TME becomes increasingly essential to every aspect of cancer research and treatment. Novel spatially-resolved high-plex molecular profiling technologies have been emerging rapidly as powerful tools to obtain in-depth understanding from TME perspectives due to their capacity to allow high-plex protein and RNA profiling while keeping valuable spatial information. Based on our practical experience, we review a variety of available spatial proteogenomic technologies, including 10X Visium, GeoMx Digital Spatial Profiler (DSP), cyclic immunofluorescence-based CODEX and Multi-Omyx, mass spectrometry (MS)-based imaging mass spectrometry (IMS) and multiplex ion-beam imaging (MIBI). We also discuss FISSEQ, MERFISH, Slide-seq, and HDST, some of which may become commercially available in the near future. In particular, with our experience, we elaborate on DSP for spatial proteogenomic profiling and discuss its unique features designed for immuno-oncology and propose anticipation towards its future direction. The emerging spatially technologies are rapidly reshaping the magnitude of our understanding of the TME.
肿瘤微环境（TME）蕴藏着异质性内容，并在肿瘤发生、转移和耐药性中发挥关键作用。因此，TME 的剖析对于癌症研究和治疗的各个方面变得越来越重要。新型空间分辨高多重分子分析技术作为从 TME 角度深入了解的强大工具迅速涌现，因为它们能够在保持有价值的空间信息的同时，进行高多重蛋白质和 RNA 分析。基于我们的实践经验，我们综述了各种现有的空间蛋白质组学技术，包括 10X Visium、GeoMx Digital Spatial Profiler（DSP）、基于循环免疫荧光的 CODEX 和 Multi-Omyx、基于质谱（MS）的成像质谱（IMS）和多重离子束成像（MIBI）。我们还讨论了 FISSEQ、MERFISH、Slide-seq 和 HDST，其中一些可能在不久的将来会商业化。特别是，根据我们的经验，我们详细介绍了 DSP 用于空间蛋白质组学分析，并讨论了其针对免疫肿瘤学设计的独特功能，并对其未来方向提出了预期。新兴的空间技术正在迅速改变我们对 TME 的理解程度。
