Chrysin Induced Cell Apoptosis Through /let-7a/ Axis in Gastric Cancer Cells and Inhibited Tumor Growth.

BACKGROUND

Chrysin is a natural flavone that is present in honey and has exhibited anti-tumor properties. It has been widely studied as a therapeutic agent for the treatment of various types of cancers. The objectives of this present study were to elucidate how chrysin regulates non-coding RNA expression to exert anti-tumor effects in gastric cancer cells.

METHODS

Through the use of RNA sequencing, we investigated the differential expression of mRNAs in gastric cancer cells treated with chrysin. Furthermore, and let-7a overexpression and knockdown were conducted. Other features, including cell growth, apoptosis, migration and invasion, were also analyzed. Knockout of the gene was generated using the CRISPR/Cas9 system for tumor growth analysis .

RESULTS

Our results identified as a differentially expressed mRNA that is down-regulated following treatment with chrysin. Moreover, the results showed that chrysin can induce cellular apoptosis and inhibit cell migration and invasion. To further determine the underlying mechanism of COPB2 expression, we investigated the expression of the long non-coding RNA (lncRNA) and microRNA let-7a. Our results showed that treatment with chrysin significantly increased let-7a expression and reduced the expression of and . In addition, our results demonstrated that reduced expression of markedly promotes cell apoptosis. Finally, data suggested that expression is related to tumor growth.

CONCLUSIONS

This study suggests that chrysin exhibited anti-tumor effects through a /let-7a/ axis.