Chen Lin, Li Qirong, Jiang Ziping, Li Chengshun, Hu Haobo, Wang Tiedong, Gao Yan, Wang Dongxu
Laboratory Animal Center, College of Animal Science, Jilin University, Changchun, China.
Department of Hand Surgery, The First Hospital of Jilin University, Changchun, China.
Front Oncol. 2021 Jun 3;11:651644. doi: 10.3389/fonc.2021.651644. eCollection 2021.
Chrysin is a natural flavone that is present in honey and has exhibited anti-tumor properties. It has been widely studied as a therapeutic agent for the treatment of various types of cancers. The objectives of this present study were to elucidate how chrysin regulates non-coding RNA expression to exert anti-tumor effects in gastric cancer cells.
Through the use of RNA sequencing, we investigated the differential expression of mRNAs in gastric cancer cells treated with chrysin. Furthermore, and let-7a overexpression and knockdown were conducted. Other features, including cell growth, apoptosis, migration and invasion, were also analyzed. Knockout of the gene was generated using the CRISPR/Cas9 system for tumor growth analysis .
Our results identified as a differentially expressed mRNA that is down-regulated following treatment with chrysin. Moreover, the results showed that chrysin can induce cellular apoptosis and inhibit cell migration and invasion. To further determine the underlying mechanism of COPB2 expression, we investigated the expression of the long non-coding RNA (lncRNA) and microRNA let-7a. Our results showed that treatment with chrysin significantly increased let-7a expression and reduced the expression of and . In addition, our results demonstrated that reduced expression of markedly promotes cell apoptosis. Finally, data suggested that expression is related to tumor growth.
This study suggests that chrysin exhibited anti-tumor effects through a /let-7a/ axis.
白杨素是一种天然黄酮，存在于蜂蜜中，并已显示出抗肿瘤特性。它作为一种治疗多种癌症的治疗剂已被广泛研究。本研究的目的是阐明白杨素如何调节非编码RNA表达以在胃癌细胞中发挥抗肿瘤作用。
通过使用RNA测序，我们研究了用白杨素处理的胃癌细胞中mRNA的差异表达。此外，进行了let-7a的过表达和敲低。还分析了其他特征，包括细胞生长、凋亡、迁移和侵袭。使用CRISPR/Cas9系统敲除该基因以进行肿瘤生长分析。
我们的结果确定了作为一种差异表达的mRNA，在用白杨素处理后其表达下调。此外，结果表明白杨素可诱导细胞凋亡并抑制细胞迁移和侵袭。为了进一步确定COPB2表达的潜在机制，我们研究了长链非编码RNA（lncRNA）和微小RNA let-7a的表达。我们的结果表明，用白杨素处理可显著增加let-7a的表达并降低和的表达。此外，我们的结果表明的表达降低明显促进细胞凋亡。最后，数据表明的表达与肿瘤生长有关。
本研究表明白杨素通过/let-7a/轴发挥抗肿瘤作用。
