Betjes Max A, Zheng Xuan, Kok Rutger N U, van Zon Jeroen S, Tans Sander J
AMOLF, Amsterdam, Netherlands.
Bionanoscience Department, Kavli Institute of Nanoscience Delft, Delft University of Technology, Delft, Netherlands.
Front Cell Dev Biol. 2021 Jun 3;9:675013. doi: 10.3389/fcell.2021.675013. eCollection 2021.
Organoids have emerged as powerful model systems to study organ development and regeneration at the cellular level. Recently developed microscopy techniques that track individual cells through space and time hold great promise to elucidate the organizational principles of organs and organoids. Applied extensively in the past decade to embryo development and 2D cell cultures, cell tracking can reveal the cellular lineage trees, proliferation rates, and their spatial distributions, while fluorescent markers indicate differentiation events and other cellular processes. Here, we review a number of recent studies that exemplify the power of this approach, and illustrate its potential to organoid research. We will discuss promising future routes, and the key technical challenges that need to be overcome to apply cell tracking techniques to organoid biology.
类器官已成为在细胞水平上研究器官发育和再生的强大模型系统。最近开发的能够在空间和时间上追踪单个细胞的显微镜技术，对于阐明器官和类器官的组织原理具有巨大潜力。在过去十年中，细胞追踪技术广泛应用于胚胎发育和二维细胞培养，它可以揭示细胞谱系树、增殖率及其空间分布，而荧光标记则可指示分化事件和其他细胞过程。在这里，我们回顾了一些近期研究，这些研究例证了这种方法的强大之处，并阐述了其在类器官研究中的潜力。我们将讨论未来有前景的路线，以及将细胞追踪技术应用于类器官生物学需要克服的关键技术挑战。
