Fuzhou Center for Disease Control and Prevention, Fuzhou, 350004, Fujian, People's Republic of China.
Fujian Medical University, Fuzhou, 350004, Fujian, People's Republic of China.
Arch Microbiol. 2021 Sep;203(7):4579-4585. doi: 10.1007/s00203-021-02441-0. Epub 2021 Jun 22.
A one-step 3 h melting curve analysis-based technology for spacer oligonucleotide typing (McSpoligotyping) analyzed with the SITVIT2 database was applied to detect epidemiological events of Mycobacterium tuberculosis (MTB) in a coastal city of China from 2016 to 2018. 306 MTB isolates were identified by Fuzhou tuberculosis designated hospitals between 2016 and 2018. The results showed that the MTB isolates were divided into Beijing family and non-Beijing families, accounting for 45.42% (139/306) and 54.58% (167/306), respectively. H and T families were the prevalent genotypes in non-Beijing families. Herein, the spoligotyping technology has practical application for the classification and tracing of tuberculosis in the public.
一种基于一步 3 小时熔解曲线分析的 spacer 寡核苷酸分型（McSpoligotyping）技术，结合 SITVIT2 数据库，用于检测中国沿海城市 2016 年至 2018 年结核分枝杆菌（MTB）的流行病学事件。2016 年至 2018 年间，福州结核病定点医院共鉴定出 306 株 MTB 分离株。结果表明，MTB 分离株分为北京家族和非北京家族，分别占 45.42%（139/306）和 54.58%（167/306）。H 和 T 家族是非北京家族中流行的基因型。因此， spoligotyping 技术在结核病的分类和溯源方面具有实际应用价值。
