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利用基于连接的扩增和熔解曲线分析对结核分枝杆菌复合群分离株进行寡核苷酸分型

Spoligotyping of Mycobacterium tuberculosis Complex Isolates by Use of Ligation-Based Amplification and Melting Curve Analysis.

作者信息

Zeng Xiaohong, Li Hui, Zheng Rongrong, Kurepina Natalia, Kreiswirth Barry N, Zhao Xilin, Xu Ye, Li Qingge

机构信息

Engineering Research Centre of Molecular Diagnostics, Ministry of Education, State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian, China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Public Health, Xiamen University, Xiamen, Fujian, China Engineering Research Centre of Personalized Molecular Diagnostics of Xiamen, Fujian, China.

Tuberculosis Reference Laboratory, Henan Center for Disease Control and Prevention, Zhengzhou, Henan, China.

出版信息

J Clin Microbiol. 2016 Sep;54(9):2384-7. doi: 10.1128/JCM.00857-16. Epub 2016 Jun 22.

DOI:10.1128/JCM.00857-16
PMID:27335152
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5005503/
Abstract

We report here a ligation-based spoligotyping that can identify unamplified spacers in membrane-based spoligotyping due to asymmetric insertion of IS6110 in the direct repeat locus. Our typing yielded 84.4% (411/487) concordance with traditional typing and 100% (487/487) accuracy when confirmed by DNA sequencing.

摘要

我们在此报告一种基于连接的间隔寡核苷酸分型方法，该方法可识别基于膜的间隔寡核苷酸分型中未扩增的间隔区，这是由于IS6110在直接重复序列位点的不对称插入所致。我们的分型结果与传统分型的一致性为84.4%（411/487），经DNA测序确认后准确率为100%（487/487）。

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J Clin Microbiol. 2015 Dec;53(12):3876-80. doi: 10.1128/JCM.01824-15. Epub 2015 Aug 5.
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Spoligotyping of Mycobacterium tuberculosis complex isolates using hydrogel oligonucleotide microarrays.使用水凝胶寡核苷酸微阵列对结核分枝杆菌复合群分离株进行间隔寡核苷酸分型。
Infect Genet Evol. 2014 Aug;26:41-6. doi: 10.1016/j.meegid.2014.04.024. Epub 2014 May 9.
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Use of Luminex MagPlex magnetic microspheres for high-throughput spoligotyping of Mycobacterium tuberculosis isolates in Port-au-Prince, Haiti.海地太子港结核分枝杆菌分离株高通量 spoligotyping 中使用 Luminex MagPlex 磁珠。
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Combination of fluorescence color and melting temperature as a two-dimensional label for homogeneous multiplex PCR detection.荧光颜色和熔点的组合作为均相多重 PCR 检测的二维标记。
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SpolPred: rapid and accurate prediction of Mycobacterium tuberculosis spoligotypes from short genomic sequences.SpolPred：从短基因组序列中快速准确地预测结核分枝杆菌 spoligotypes。
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