Yardley-Jones A, Anderson D, Jenkinson P
Occupational Health Division, London, UK.
Br J Ind Med. 1988 Aug;45(8):516-22. doi: 10.1136/oem.45.8.516.
This study was carried out to investigate the effects of potential low level exposure to benzene on phytohaemagglutinin (PHA) stimulated lymphocytes. Sixty six male workers of a refinery population were studied and compared with 33 control workers in the same refinery who were not known to have been exposed to benzene. The responsiveness of the lymphocyte to PHA as a measure of blastogenesis was measured by the incorporation of radio labelled thymidine by the stimulated lymphocytes in vitro. Questionnaires were used to determine various lifestyle factors such as smoking, drinking, and exposure to ionising radiation. The results showed that there was no difference between the exposed group (mean 28928 + 1524 SE (decays per minute (DPM] as compared with the control group (mean 28304 + 2483 SE DPM). Furthermore, it was not possible to determine any effects attributable to various social factors. There was, however, a suggestion of a decrease in mitogenic response with age in both exposed and control workers that was consistent with other studies. It has been shown that products of benzene metabolism may affect the mitogenic response of lymphocytes in a similar way to known promoting agents. This study was unable to show these effects, probably as a result of the low exposures encountered by the individuals.
本研究旨在调查潜在的低水平苯暴露对植物血凝素（PHA）刺激的淋巴细胞的影响。对一家炼油厂的66名男性工人进行了研究，并与该厂33名已知未接触过苯的对照工人进行了比较。通过体外受刺激淋巴细胞掺入放射性标记的胸腺嘧啶核苷来测量淋巴细胞对PHA的反应性，以此作为细胞增殖的指标。使用问卷调查来确定各种生活方式因素，如吸烟、饮酒和接触电离辐射情况。结果显示，暴露组（平均28928 + 1524标准误[每分钟衰变数（DPM）]）与对照组（平均28304 + 2483标准误DPM）之间没有差异。此外，无法确定各种社会因素所产生的任何影响。然而，暴露组和对照组工人的有丝分裂反应均有随年龄下降的趋势，这与其他研究结果一致。研究表明，苯代谢产物可能以与已知促癌剂类似的方式影响淋巴细胞的有丝分裂反应。本研究未能显示出这些影响，可能是由于个体接触水平较低所致。
