Wu Yajun, Luo Xiangdong, Feng Yuliang, Yang Jiasong, Fan Hua, Cen Xiaobo, Li Wensheng
Aier School of Ophthalmology, Central South University, Changsha, Hunan, China.
Department of Ophthalmology, Shanghai Aier Eye Ophthalmology Hospital, Shanghai, China.
Front Neurosci. 2023 Jan 6;16:1106904. doi: 10.3389/fnins.2022.1106904. eCollection 2022.
Obtaining accurate axial length (AL) is very important for the establishment of animal models of myopia. The purpose of this study is to compare the accuracy of Quantel A-B scan, OD-1 A scan, and vernier caliper in measuring AL in Sprague Dawley (SD) rats.
In total, 60 5-week-old SD rats were divided into female rat group ( = 30) and male rat group ( = 30). Quantel A-B scan and OD-1 A scan were, respectively, used to measure the AL of both eyes of each living rat, and vernier caliper was used to measure the anterior-posterior diameter of each rat's eyeball. Besides, the correlation between refractive error (RE) and AL measured by different instruments was evaluated, and the accuracy of the three measurement methods was compared according to gender and left/right eyes.
There were significant differences in AL and diopter of SD rats at the same age ( < 0.05). the AL of male rats was greater than that of female rats, while diopter (D) was the opposite; There was no significant difference in AL and D between left and right eyes in the same SD rats ( > 0.05); There were statistical differences among the three measurement methods ( < 0.05), AL measured by vernier caliper was the largest, followed by Quantel A-B scan, OD-1 A scan; Difference in AL between male and female was not statistically significant between the results obtained by Quantel A-B scan and vernier caliper ( > 0.05), but there were statistically significant differences between the other two measurement methods ( < 0.05).
Sex is the influencing factor of AL and RE. Imaging measurement can accurately measure the AL in living small rodents. Compared with OD-1 A scan, Quantel A-B scan may be more accurate.
获取准确的眼轴长度（AL）对于建立近视动物模型非常重要。本研究的目的是比较Quantel A-B扫描、OD-1 A扫描和游标卡尺在测量斯普拉格-道利（SD）大鼠眼轴长度方面的准确性。
总共60只5周龄的SD大鼠被分为雌性大鼠组（n = 30）和雄性大鼠组（n = 30）。分别使用Quantel A-B扫描和OD-1 A扫描测量每只活鼠双眼的眼轴长度，并用游标卡尺测量每只大鼠眼球的前后径。此外，评估不同仪器测量的屈光不正（RE）与眼轴长度之间的相关性，并根据性别和左右眼比较三种测量方法的准确性。
同一年龄的SD大鼠眼轴长度和屈光度存在显著差异（P < 0.05）。雄性大鼠的眼轴长度大于雌性大鼠，而屈光度（D）则相反；同一SD大鼠左右眼的眼轴长度和屈光度无显著差异（P > 0.05）；三种测量方法之间存在统计学差异（P < 0.05），游标卡尺测量的眼轴长度最大，其次是Quantel A-B扫描，OD-1 A扫描最小；Quantel A-B扫描和游标卡尺测量结果中，雄性和雌性之间眼轴长度的差异无统计学意义（P > 0.05），但其他两种测量方法之间存在统计学差异（P < 0.05）。
性别是影响眼轴长度和屈光不正的因素。成像测量可以准确测量活体小型啮齿动物的眼轴长度。与OD-1 A扫描相比，Quantel A-B扫描可能更准确。
