Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
Nano Medical Engineering Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
Adv Sci (Weinh). 2021 Aug;8(16):e2100961. doi: 10.1002/advs.202100961. Epub 2021 Jun 26.
Artificial materials have no biological functions, but they are important for medical devices such as artificial organs and matrices for regenerative medicine. In this study, mitogenic and differentiation-inducible materials are devised via the simple coating of polypeptides, which contain the sequence of epidermal growth factor or insulin-like growth factor with a key amino acid (3,4-dihydroxyphenylalanine) of underwater adhesive proteins. The adhesive polypeptides prepared via solid-phase synthesis form layers on various substrates involving organic and inorganic materials to provide biological surfaces. Through the direct activation of cognate receptors on interactive surfaces, the materials enable increased cell growth and differentiation compared to that achieved by soluble growth factors. This superior growth and differentiation are attributed to the long-lasting signal transduction (triggered by the bound growth factors), which do not cause receptor internalization and subsequent downregulation.
人工材料没有生物功能，但它们对于医疗器械如人工器官和再生医学基质非常重要。在这项研究中，通过简单地涂覆含有表皮生长因子或胰岛素样生长因子序列的多肽来设计有丝分裂原和诱导分化的材料，这些多肽含有水下黏附蛋白的关键氨基酸（3,4-二羟基苯丙氨酸）。通过固相合成制备的黏附多肽在各种涉及有机和无机材料的基底上形成层，提供生物表面。通过在交互表面上直接激活同源受体，与可溶性生长因子相比，这些材料能够促进细胞的生长和分化。这种优越的生长和分化归因于持久的信号转导（由结合的生长因子触发），不会导致受体内化和随后的下调。
