He Wenqiao, Gao Yuhan, Wen Yuqi, Ke Xuemei, Ou Zejin, Li Yongzhi, He Huan, Chen Qing
Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, Guangzhou, China.
Xiamen Center for Disease Control and Prevention, Xiamen, China.
Front Microbiol. 2021 Jun 8;12:653873. doi: 10.3389/fmicb.2021.653873. eCollection 2021.
Hepatitis is a major global health concern. However, the etiology of 10-20% hepatitis cases remains unclear. Some hepatitis-associated viruses, like the hepatitis E virus, are zoonotic pathogens. Rats, shrews, and bats are reservoirs for many zoonotic pathogens. Therefore, understanding the virome in the liver of these animals is important for the investigation of the etiologies of hepatitis and monitoring the emerging zoonotic viruses. In this study, viral metagenomics and PCR methods were used to investigate viral communities in rats, mice, house shrews, and bats livers. Viral metagenomic analysis showed a diverse set of sequences in liver samples, comprising: sequences related to herpesviruses, orthomyxoviruses, anelloviruses, hepeviruses, hepadnaviruses, flaviviruses, parvoviruses, and picornaviruses. Using PCR methods, we first detected hepatovirus sequences in (3.85%). We also reported the first detection of Zika virus-related sequences in rats and house shrews. Sequences related to influenza A virus and herpesviruses were detected in liver. Higher detection rates of pegivirus sequences were found in liver tissue and serum samples from rats (7.85% and 15.79%, respectively) than from house shrews. Torque teno virus sequences had higher detection rates in the serum samples of rats and house shrews (52.72% and 5.26%, respectively) than in the liver. Near-full length genomes of pegivirus and torque teno virus were amplified. This study is the first to compare the viral communities in the liver of bats, rats, mice, and house shrews. Its findings expand our understanding of the virome in the liver of these animals and provide an insight into hepatitis-related viruses.
肝炎是一个重大的全球健康问题。然而,10%-20%的肝炎病例病因仍不清楚。一些与肝炎相关的病毒,如戊型肝炎病毒,是人畜共患病原体。大鼠、鼩鼱和蝙蝠是许多人畜共患病原体的宿主。因此,了解这些动物肝脏中的病毒群落对于调查肝炎病因和监测新出现的人畜共患病病毒非常重要。在本研究中,采用病毒宏基因组学和PCR方法调查大鼠、小鼠、家鼩鼱和蝙蝠肝脏中的病毒群落。病毒宏基因组分析显示肝脏样本中有多种序列,包括:与疱疹病毒、正粘病毒、环病毒、庚型肝炎病毒、嗜肝DNA病毒、黄病毒、细小病毒和小RNA病毒相关的序列。使用PCR方法,我们首次在(3.85%)中检测到肝病毒序列。我们还首次报告在大鼠和家鼩鼱中检测到寨卡病毒相关序列。在肝脏中检测到与甲型流感病毒和疱疹病毒相关的序列。在大鼠的肝脏组织和血清样本中,戊型肝炎病毒相关序列的检出率(分别为7.85%和15.79%)高于家鼩鼱。大鼠和家鼩鼱血清样本中TTV序列的检出率(分别为52.72%和5.26%)高于肝脏。扩增了戊型肝炎病毒和TTV的近全长基因组。本研究首次比较了蝙蝠、大鼠、小鼠和家鼩鼱肝脏中的病毒群落。其研究结果扩展了我们对这些动物肝脏中病毒群落的理解,并为与肝炎相关的病毒提供了见解。