使用窄径柱和二极管阵列检测的高效液相色谱法对法医纤维分析中的酸性染料进行表征。

Characterisation of acid dyes in forensic fibre analysis by high-performance liquid chromatography using narrow-bore columns and diode array detection.

作者信息

Laing D K, Gill R, Blacklaws C, Bickley H M

机构信息

Central Research Establishment, Home Office Forensic Science Service, Reading, Berkshire, U.K.

出版信息

J Chromatogr. 1988 Jun 17;442:187-208. doi: 10.1016/s0021-9673(00)94468-6.

DOI:10.1016/s0021-9673(00)94468-6

PMID:3417814

Abstract

A gradient elution high-performance liquid chromatographic (HPLC) system with a diode array detector and a short narrow-bore (40 x 1 mm I.D.) column has been used to characterise a number of acid dyes. The resolution and reproducibility of the HPLC system have been evaluated and the detection limits for various dyes have been estimated. Comparisons are made with current methods of fibre dyestuff examination used in forensic science. The system has been applied to the analysis of dye extracted from single fibres. Using diode array detection, both chromatographic and spectral data can be produced in a single operation from casework sized samples.

摘要

一种配备二极管阵列检测器和短窄内径（40×1毫米内径）色谱柱的梯度洗脱高效液相色谱（HPLC）系统已被用于表征多种酸性染料。对该HPLC系统的分辨率和重现性进行了评估，并估算了各种染料的检测限。与法医学中目前使用的纤维染料检测方法进行了比较。该系统已应用于单根纤维中提取的染料的分析。使用二极管阵列检测，可以在一次操作中从实际案件大小的样本中生成色谱和光谱数据。