Jairak Waleemas, Charoenkul Kamonpan, Chamsai Ekkapat, Udom Kitikhun, Chaiyawong Supassama, Hangsawek Aphinya, Waenkaew Suchart, Mungaomklang Anek, Tangwangvivat Ratanaporn, Amonsin Alongkorn
Faculty of Veterinary Science, Center of Excellence for Emerging and Re-emerging Infectious Diseases in Animals, and One Health Research Cluster, Chulalongkorn University, Bangkok, Thailand.
Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Zoonoses Public Health. 2022 Sep;69(6):737-745. doi: 10.1111/zph.12907. Epub 2022 Jan 4.
A cross-sectional survey of SARS-CoV-2 in domestic dogs and cats was conducted in high-risk areas, five subdistricts of Samut Sakhon Province, the epicenter of the second wave of the COVID-19 outbreak in Thailand in February 2021. A total of 523 swab samples (nasal, oral, and rectal swabs) and 159 serum samples from dogs (n = 83) and cats (n = 93) were collected and tested for SARS-CoV-2 RNA and antibodies. All swab samples tested negative for SARS-CoV-2 RNA by real-time RT-PCR with three panels of specific primers and probes. Although all dogs and cats were negative for SARS-CoV-2 RNA, 3.14% (5/159) had anti-N-IgG antibodies against SARS-CoV-2 by indirect multispecies ELISA. Our results demonstrated SARS-CoV-2 exposure in domestic animals living in high-risk areas during the second wave of the COVID-19 outbreak in Thailand. Thus, the use of one health approach for monitoring SARS-CoV-2 in domestic animals in high-risk areas of COVID-19 outbreaks should be routinely conducted and will provide benefits to risk communications in communities.
2021年2月，泰国第二波新冠疫情的中心是北榄府的五个分区，在这些高风险地区对家犬和家猫进行了SARS-CoV-2横断面调查。共采集了523份拭子样本（鼻拭子、口拭子和直肠拭子）以及159份来自犬（n = 83）和猫（n = 93）的血清样本，检测SARS-CoV-2 RNA和抗体。所有拭子样本通过使用三组特异性引物和探针的实时RT-PCR检测，SARS-CoV-2 RNA均呈阴性。尽管所有犬猫的SARS-CoV-2 RNA均为阴性，但通过间接多物种ELISA检测，3.14%（5/159）的动物具有抗SARS-CoV-2的N-IgG抗体。我们的结果表明，在泰国第二波新冠疫情期间，高风险地区的家畜接触过SARS-CoV-2。因此，应定期采用“同一健康”方法监测新冠疫情高风险地区家畜中的SARS-CoV-2，这将有助于社区的风险沟通。
