Blair A L, Cree I A, Beck J S, Hastings M J
Department of Pathology, University of Dundee, Ninewells Hospital and Medical School, Scotland, U.K.
J Immunol Methods. 1988 Sep 13;112(2):163-8. doi: 10.1016/0022-1759(88)90352-3.
Previously described assays of phagocyte chemiluminescence have required large numbers of cells and have not been able to follow responses from a large number of samples in single experiments. Recently, sensitive luminometers which employ a 96 well microtitre plate format have become available. We describe the application of this equipment to the measurement of phagocyte chemiluminescence using lucigenin to enhance the response and the estimation of the opsonic activity of serum. It was found that as few as 5 X 10(4) cells (polymorphonuclear leukocytes or monocytes) per well and a ratio of 10:1 zymosan particles to cells gave good results when opsonised with 10% whole serum. This method allows assays of opsonic activity to be performed in triplicate on large numbers of sera with a relatively small number of phagocytes and should aid the investigation of the role of opsonisation in infectious disease.
先前描述的吞噬细胞化学发光检测方法需要大量细胞，并且无法在单个实验中跟踪大量样本的反应。最近，采用96孔微量滴定板形式的灵敏发光计已经问世。我们描述了该设备在使用光泽精增强反应来测量吞噬细胞化学发光以及评估血清调理活性方面的应用。结果发现，当用10%全血清进行调理时，每孔低至5×10⁴个细胞（多形核白细胞或单核细胞）以及酵母聚糖颗粒与细胞的比例为10:1时可获得良好结果。该方法允许用相对少量的吞噬细胞对大量血清进行调理活性检测，并一式三份进行，这应该有助于研究调理作用在传染病中的作用。
