Department of Pharmacy Practice, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Department of Urology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
In Vivo. 2021 Jul-Aug;35(4):2073-2080. doi: 10.21873/invivo.12476.
BACKGROUND/AIM: Little is known on urine biomarkers that are associated with malignant behavior in patients with bladder cancer (BC). Our aim was to identify BC-related factors in urine samples using our original method "immune complexome analysis", based on detecting the immune complex (IC).
Immune complexome analysis was performed using urine samples from 97 BC patients, including 67 with non-muscle invasive BC (NMIBC).
Eight IC-antigens were recognized as candidates for BC-related factors from 20,165 proteins. IC-serum albumin, -fibrinogen γ chain, -hemoglobin subunit α, -hemoglobin subunit β, -ceruloplasmin, and fibrinogen β chain were significantly associated with either pathological features and/or outcome. IC-ceruloplasmin was most widely associated with pathological features in all BC patients and lamina propria invasion and urinary tract recurrence in NMIBC.
Based on detection of IC-antigens it was demonstrated that six IC-antigens, especially IC-ceruloplasmin, are potential urine biomarkers in BC.
背景/目的：膀胱癌（BC）患者的尿液生物标志物与恶性行为相关的信息知之甚少。我们的目的是使用我们的原始方法“免疫复合物组分析”，基于检测免疫复合物（IC），来鉴定尿液样本中的 BC 相关因素。
对 97 例 BC 患者的尿液样本进行了免疫复合物组分析，其中包括 67 例非肌肉浸润性 BC（NMIBC）患者。
从 20165 种蛋白质中鉴定出 8 种 IC-抗原作为 BC 相关因素的候选物。IC-血清白蛋白、-纤维蛋白原γ链、-血红蛋白亚基α、-血红蛋白亚基β、-铜蓝蛋白和纤维蛋白原β链与病理特征和/或预后显著相关。IC-铜蓝蛋白与所有 BC 患者的病理特征以及 NMIBC 的固有层浸润和尿路复发最广泛相关。
基于 IC-抗原的检测表明，六种 IC-抗原，特别是 IC-铜蓝蛋白，是 BC 潜在的尿液生物标志物。
