Food Security and Safety Niche Area, Faculty of Natural and Agricultural Sciences, North-West University, Private Bag X2046, Mmabatho, 2735, South Africa.
Curr Genet. 2021 Dec;67(6):891-907. doi: 10.1007/s00294-021-01199-8. Epub 2021 Jun 30.
Diverse agriculturally important microbes have been studied with known potential in plant growth promotion. Providing several opportunities, Stenotrophomonas species are characterized as promising plant enhancers, inducers, and protectors against environmental stressors. The S. indicatrix BOVIS40 isolated from the sunflower root endosphere possessed unique features, as genome insights into the Stenotrophomonas species isolated from oilseed crops in Southern Africa have not been reported. Plant growth-promotion screening and genome analysis of S. indicatrix BOVIS40 were presented in this study. The genomic information reveals various genes underlining plant growth promotion and resistance to environmental stressors. The genome of S. indicatrix BOVIS40 harbors genes involved in the degradation and biotransformation of organic molecules. Also, other genes involved in biofilm production, chemotaxis, and flagellation that facilitate bacterial colonization in the root endosphere and phytohormone genes that modulate root development and stress response in plants were detected in strain BOVIS40. IAA activity of the bacterial strain may be a factor responsible for root formation. A measurable approach to the S. indicatrix BOVIS40 lifestyle can strategically provide several opportunities in their use as bioinoculants in developing environmentally friendly agriculture sustainably. The findings presented here provide insights into the genomic functions of S. indicatrix BOVIS40, which has set a foundation for future comparative studies for a better understanding of the synergism among microbes inhabiting plant endosphere. Hence, highlighting the potential of S. indicatrix BOVIS40 upon inoculation under greenhouse experiment, thus suggesting its application in enhancing plant and soil health sustainably.
已对多种具有促进植物生长潜力的重要农业微生物进行了研究。茎单胞菌属的一些物种具有促进植物生长、诱导和保护植物免受环境胁迫的潜力，被认为是有前途的植物增强剂、诱导剂和保护剂。从向日葵根内生区分离得到的指示短杆菌 BOVIS40 具有独特的特征，因为尚未报道过从南非油籽作物中分离得到的茎单胞菌属物种的基因组分析。本研究对指示短杆菌 BOVIS40 进行了植物促生筛选和基因组分析。基因组信息揭示了各种与植物生长促进和抵抗环境胁迫有关的基因。指示短杆菌 BOVIS40 的基因组包含参与有机分子降解和生物转化的基因。此外，还检测到与生物膜形成、趋化性和鞭毛有关的其他基因，这些基因有助于细菌在根内生区的定植，以及调节植物根发育和应激反应的植物激素基因。该菌株的 IAA 活性可能是导致根形成的一个因素。对指示短杆菌 BOVIS40 生活方式的可衡量方法可以为其作为生物接种剂在可持续发展的环保农业中的应用提供多种战略机会。这里提出的发现提供了对指示短杆菌 BOVIS40 基因组功能的深入了解，为进一步研究微生物共生关系奠定了基础。因此，在温室实验中接种后突出了指示短杆菌 BOVIS40 的潜力，从而表明其在可持续增强植物和土壤健康方面的应用。
